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RACK1/Asc1p, a ribosomal node in cellular signaling.

Nicole Rachfall1, Kerstin Schmitt, Susanne Bandau

  • 1Institute of Microbiology and Genetics, Georg-August Universität, D-37077 Göttingen, Germany.

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Deleting ASC1 in yeast impacts iron uptake, causes stress, and shifts metabolism. Ribosomal Asc1p regulates signaling pathways and transcription factors, affecting cellular responses.

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Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Yeast Genetics

Background:

  • RACK1/Asc1p orthologues are involved in diverse cellular signaling pathways.
  • The complete deletion effects of ASC1 have not been comprehensively studied.
  • Understanding Asc1p's role is crucial for comprehending eukaryotic cellular processes.

Purpose of the Study:

  • To comprehensively analyze the cellular processes affected by ASC1 deletion in Saccharomyces cerevisiae.
  • To investigate the impact of ASC1 deletion on proteome, transcriptome, and cellular phenotype.
  • To elucidate Asc1p's regulatory role in signal transduction and metabolism.

Main Methods:

  • De novo proteome and transcriptome analysis of Saccharomyces cerevisiae Δasc1 deletion strain.
  • Phenotypical characterization of the Δasc1 mutant.
  • Analysis of MAP kinase signal transduction pathways and transcription factor regulation.

Main Results:

  • ASC1 deletion reduces iron uptake and induces nitrosative stress, indicative of hypoxia.
  • A metabolic shift from respiration to fermentation occurs in the Δasc1 strain.
  • Aberrant cellular responses in Δasc1 are linked to altered transcription factor abundances and translational regulation.

Conclusions:

  • Ribosomal Asc1p is a key regulator in cellular signaling pathways, including those for invasive growth and cell wall integrity.
  • Asc1p influences cellular metabolism and stress responses, potentially through translational control of transcription factors.
  • The study highlights Asc1p's multifaceted role in maintaining cellular homeostasis and response to environmental cues.