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Complement System

Complement System

The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a membrane...

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Related Experiment Video

Updated: May 17, 2026

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

Published on: January 29, 2014

Functional analyses of complement convertases using C3 and C5-depleted sera.

Marcin Okroj¹, Emelie Holmquist, Ben C King

¹Department of Laboratory Medicine, Lund University, The Wallenberg Laboratory, Malmö, Sweden.

|October 17, 2012

Summary

This summary is machine-generated.

This study introduces a new method using depleted sera to assess complement convertase activity and regulation. The novel assay simplifies the study of complement inhibitors like thioredoxin-1 on convertase formation and stability.

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Last Updated: May 17, 2026

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

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Published on: January 29, 2014

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Depletion of Specific Cell Populations by Complement Depletion

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Published on: February 5, 2010

Area of Science:

Immunology
Biochemistry

Background:

Complement cascade central stages involve C3 and C5 convertases.
Convertases amplify complement activation and form the membrane attack complex.
Existing assays using purified components risk non-physiological interactions.

Purpose of the Study:

To develop a novel method for studying complement convertase formation and stability.
To assess the influence of putative regulators on convertase activity in a more physiological context.
To investigate the mechanism of thioredoxin-1's effect on complement convertases.

Main Methods:

Utilized C3 or C5-depleted sera to support complement cascade activation.
Developed a method for assessing convertase formation and stability.
Applied the assay to study thioredoxin-1's influence on complement convertases.

Main Results:

The novel method allows fast and simple assessment of convertase activity.
Demonstrated practical utility by studying thioredoxin-1.
Provided insights into thioredoxin-1's mechanism of action on complement convertases.

Conclusions:

The developed assay offers a valuable alternative for studying complement convertases.
This method enhances the physiological relevance of complement regulator studies.
Further research can utilize this assay to explore various complement inhibitors.