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Related Experiment Video

Updated: May 17, 2026

High-throughput Protein Expression Generator Using a Microfluidic Platform
09:26

High-throughput Protein Expression Generator Using a Microfluidic Platform

Published on: August 23, 2012

Functional protein microarrays by electrohydrodynamic jet printing.

Kazuyo Shigeta1, Ying He, Erick Sutanto

  • 1Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.

Analytical Chemistry
|October 18, 2012
PubMed
Summary

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Advanced electrohydrodynamic jet (e-jet) printing precisely patterns proteins on diverse surfaces without altering their function. This technology enables rapid, large-area micro/nanoscale arrays for biotechnology applications.

Area of Science:

  • Biotechnology
  • Materials Science
  • Surface Chemistry

Background:

  • Micro- and nanoscale patterning of biomolecules is crucial for advanced analytical platforms.
  • Existing methods face challenges in versatility, precision, and biomolecule integrity.

Purpose of the Study:

  • To report advanced electrohydrodynamic jet (e-jet) printing for creating micro- and nanoscale protein patterns.
  • To demonstrate the compatibility of e-jet printing with various proteins and substrates.
  • To validate the preservation of protein structure and function after printing.

Main Methods:

  • Utilized advanced electrohydrodynamic jet (e-jet) printing techniques.
  • Patterned diverse proteins (streptavidin, IgG, fibrinogen, γ-globulin) on silica and plasmonic crystal substrates.

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Last Updated: May 17, 2026

High-throughput Protein Expression Generator Using a Microfluidic Platform
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Counting Proteins in Single Cells with Addressable Droplet Microarrays
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Microcontact Printing of Proteins for Cell Biology

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  • Employed multinozzle systems for multiplexed protein patterning and high-speed pulsed modes for large-area printing.

    • Main Results:

    • Achieved precise micro- and nanoscale protein patterning on flat and structured surfaces.
    • Demonstrated no adverse effects on protein structure or function, exemplified by streptavidin's specific binding.
    • Enabled rapid, large-area printing of millions of droplets with controlled size and spacing.
    • Showcased compatibility with plasmonic crystals for label-free detection.

    Conclusions:

    • Advanced e-jet printing offers a versatile and precise method for fabricating protein micro/nanoarrays.
    • The technique preserves biomolecular integrity, crucial for functional assays.
    • Its compatibility with diverse substrates and detection modes highlights broad potential in biotechnology and diagnostics.