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Updated: May 17, 2026
Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts
Published on: October 21, 2022
Optical coherence tomography and ultrasound biomicroscopy imaging of opaque corneas
Shi-you Zhou1, Chun-xiao Wang, Xiao-yu Cai
1Corneal Division, Zhongshan Ophthalmic Center at Sun Yat-sen University, The State Key Laboratory of Ophthalmology, Guangzhou, China.
Purpose:
The purpose was to determine the interchangeability of ultrasound biomicroscopy (UBM) and anterior segment optical coherence tomography (AS-OCT) for corneal opacity depth measurement.
Methods:
Twenty-six eyes of 26 consecutive patients with corneal opacities were examined by both AS-OCT and UBM. The corneal thickness and the corneal opacity depth were measured and compared. The interchangeability was determined by Bland-Altman plotting.
Results:
The difference in the full corneal thickness and in the corneal opacity depth between OCT and UBM was 5 ± 7 μm and -1 ± 8 μm, respectively. There were strong correlations and no significant differences between the paired parameters (all r > 0.99, P < 0.01). The limits of agreement were 5 ± 14 μm for the corneal thickness and -1 ± 14.8 μm for the corneal opacity depth.
Conclusions:
AS-OCT and UBM may be used interchangeably for measuring both full corneal thickness and corneal opacity depth in patients with corneal opacity.
