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Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry
Published on: October 28, 2014
Measurements of cytosolic ion concentrations in live cells
Veena S Anil1, P G Kavitha, Sam Kuruvilla
1Department of (Agri) Biotechnology, College of Agriculture-Hassan, Subcampus of University of Agricultural Sciences, Bangalore, India.
Methods in Molecular Biology (Clifton, N.J.)
|October 18, 2012
Abstract:
Here, we describe a series of methods suitable for the measurement of cytosolic ion concentrations in living plant cells using ion selective dyes. We describe procedures for the use of SBFI for the measurement of Na(+) in live cells. The resulting material is suitable for most standard cell biology procedures.
