Connections between chromatin signatures and splicing
Luciana I Gómez Acuña1, Ana Fiszbein, Mariano Alló
1Laboratorio de Fisiología y Biología Molecular, Departamento de Fisiología, Biología Molecular y Celular, IFIBYNE-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.
Wiley Interdisciplinary Reviews. RNA
|October 18, 2012
Summary
Gene splicing and its regulation are crucial for human development and disease. Chromatin structure adds another layer, influencing splicing decisions and potentially creating an epigenetic memory for splicing patterns.
Area of Science:
- Molecular Biology
- Genetics
- Epigenetics
Background:
- Splicing and alternative splicing are fundamental to human gene expression, impacting cell differentiation, cycle progression, and development.
- Dysregulation of splicing is linked to various diseases, necessitating a deeper understanding of its regulatory mechanisms.
Purpose of the Study:
- To explore the intricate regulatory network of gene splicing.
- To investigate the role of chromatin structure in modulating splicing processes.
- To understand the interplay between splicing, transcription, and chromatin modifications.
Main Methods:
- Review of accumulated evidence on trans-acting factors and cis-regulatory elements.
- Analysis of cotranscriptional splicing mechanisms and transcription-associated features.
- Examination of chromatin structure's influence on RNA polymerase II elongation and splicing factor recruitment.
Main Results:
- Splicing regulation involves a complex interplay of factors, transcription dynamics, and chromatin structure.
- Chromatin modifications can influence both alternative and constitutive splicing.
- Splicing is essential for establishing specific chromatin signatures, indicating functional interconnections.
Conclusions:
- Chromatin structure represents a significant layer in splicing regulation, affecting elongation and factor recruitment.
- The functional links between chromatin and splicing suggest a potential for epigenetic memory in splicing patterns.
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