Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 17, 2026

Murine Corneal Transplantation: A Model to Study the Most Common Form of Solid Organ Transplantation

Murine Corneal Transplantation: A Model to Study the Most Common Form of Solid Organ Transplantation

Published on: November 17, 2014

Bevacizumab and rapamycin can decrease corneal opacity and apoptotic keratocyte number following photorefractive

Kyoung Sub Lee¹, Dong-Ah Ko, Eun-Soon Kim

¹Department of Ophthalmology and Biomedical Institute of Technology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.

Investigative Ophthalmology & Visual Science

|October 18, 2012

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Clinical Outcomes After Bilateral Mix-and-match Implantation of Extended Depth of Focus and Trifocal Intraocular Lenses.

Korean journal of ophthalmology : KJO·2026

Same author

A Retrospective Study: Comparative Visual Performance of Two Non-Diffractive Extended Depth-of-Focus Intraocular Lenses: TECNIS PureSee versus Clareon Vivity.

Clinical ophthalmology (Auckland, N.Z.)·2026

Same author

Therapeutic Efficacy of Rapamycin in an Experimental Mouse Model of Corneal Alkali Burn.

International journal of molecular sciences·2026

Same author

A Randomized Multicenter Study Comparing Low-Viscosity with Comparator 0.3% Hyaluronic Acid for the Treatment of Dry Eye Disease.

Ophthalmology and therapy·2026

Same author

Clinical Experience with Scleral Lens Wear After Corneal Cross-Linking in Keratoconus: Longitudinal Keratometric Outcomes.

Journal of clinical medicine·2026

Same author

High-Purity Functional Corneal Endothelial Cells From Human Induced Pluripotent Stem Cells via a Novel Wash-Out Method.

MedComm·2026

Bevacizumab and rapamycin reduce corneal haze and cell death after refractive surgery. These drugs may offer safer alternatives to mitomycin C, with less impact on corneal cells.

Area of Science:

Ophthalmology
Regenerative Medicine
Corneal Surgery

Background:

Photorefractive keratectomy (PRK) followed by UV-B irradiation can cause central corneal opacity.
Keratocyte apoptosis contributes to corneal haze and opacity post-PRK.
Mitomycin C (MMC) is used to prevent haze but can affect keratocyte survival.

Purpose of the Study:

To evaluate the efficacy of bevacizumab and rapamycin in reducing corneal opacity and keratocyte apoptosis after PRK and UV-B irradiation.
To compare the effects of bevacizumab and rapamycin with mitomycin C (MMC) and a control.

Main Methods:

Sixty Sprague-Dawley rats underwent PRK (80 μm ablation, 3-mm zone).
Groups received intraoperative application of MMC (0.02%), bevacizumab (2.5%), rapamycin (0.01%), or balanced saline solution (control) for 2 minutes.

More Related Videos

An Alkali-burn Injury Model of Corneal Neovascularization in the Mouse

An Alkali-burn Injury Model of Corneal Neovascularization in the Mouse

Published on: April 7, 2014

Retinal Pigment Epithelium Transplantation in a Non-human Primate Model for Degenerative Retinal Diseases

Retinal Pigment Epithelium Transplantation in a Non-human Primate Model for Degenerative Retinal Diseases

Published on: June 14, 2021

Related Experiment Videos

Last Updated: May 17, 2026

Murine Corneal Transplantation: A Model to Study the Most Common Form of Solid Organ Transplantation

Murine Corneal Transplantation: A Model to Study the Most Common Form of Solid Organ Transplantation

Published on: November 17, 2014

An Alkali-burn Injury Model of Corneal Neovascularization in the Mouse

An Alkali-burn Injury Model of Corneal Neovascularization in the Mouse

Published on: April 7, 2014

Retinal Pigment Epithelium Transplantation in a Non-human Primate Model for Degenerative Retinal Diseases

Retinal Pigment Epithelium Transplantation in a Non-human Primate Model for Degenerative Retinal Diseases

Published on: June 14, 2021

All eyes were exposed to UV-B irradiation (100 mJ/cm²) at 3 weeks post-PRK.

Haze, apoptotic keratocytes, and keratocyte numbers were assessed at 3, 6, and 12 weeks via biomicroscopy, TUNEL staining, and histology.

Main Results:

Bevacizumab and rapamycin significantly reduced central corneal haze and apoptotic keratocyte numbers compared to the control group at 6 and 12 weeks.
At 6 weeks, keratocyte numbers were lower in the MMC group than in the bevacizumab and rapamycin groups.
At 12 weeks, keratocyte numbers were significantly lower in the MMC group than in the rapamycin and control groups.

Conclusions:

Intraoperative bevacizumab and rapamycin effectively decrease corneal haze and keratocyte apoptosis following PRK and UV-B irradiation.
Bevacizumab and rapamycin demonstrate potential as safer alternatives to MMC in refractive surgery.
These agents may prevent postoperative corneal opacity while better preserving keratocyte populations.