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Updated: May 17, 2026

Live-cell Imaging of Lysosomal Membrane Permeabilization During Necroptosis
Published on: November 14, 2025
Oligonol-induced degradation of perilipin 1 is regulated through lysosomal degradation machinery
Junetsu Ogasawara1, Kentaro Kitadate, Hiroshi Nishioka
1Department of Molecular Predictive Medicine and Sport Science, Kyorin University, School of Medicine, Tokyo 181-8611, Japan. junetsu@ks.kyorin-u.ac.jp
Abstract:
The results obtained from our previous study showed that the addition of a lychee fruit-derived low molecular form of polyphenol, Oligonol, provoked higher levels of lipolytic activity via the degradation of perilipin 1 in primary rat adipocytes. In the current study, we investigated the possible mechanisms by which Oligonol could promote the degradation of perilipin 1 protein. The addition of Oligonol caused the degradation of GFP-tagged perilipin 1 in a time-dependent manner. Meanwhile, the co-addition of Oligonol and NH4CI, a lysosome inhibitor, failed to promote the degradation of perilipin 1, while the co-addition of Oligonol and MG132, a proteasome inhibitor, induced a reduction in the levels of perilipin 1. These results suggest that the Oligonol-induced degradation of perilipin 1 is regulated via a lysosome-dependent mechanism.
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