Related Experiment Video
Updated: May 17, 2026
Solid Lipid Nanoparticles (SLNs) for Intracellular Targeting Applications
Published on: November 17, 2015
Orally delivered salmon calcitonin-loaded solid lipid nanoparticles prepared by micelle-double emulsion method via
Chunhui Chen1, Tingting Fan, Yun Jin
1Key Laboratory of Drug Targeting & Drug Delivery System, Ministry of Education, West China School of Pharmacy, Sichuan University, Number 17 Block 3 Southern Renmin Road, Chengdu, China.
Aim:
The purpose of this study was to develop a new orally delivered nanoparticulate system to improve the bioavailability of salmon calcitonin (sCT).
Materials & Methods:
Four sCT-loaded solid lipid nanoparticles (SLNs) were prepared successfully by micelle-double emulsion technique via either the sole use of stearic acid (SA) or the combined use of SA and triglycerides (including tripalmitin [TP], trimyristin or trilaurin).
Results:
Compared with other SLNs, the combination of SA and TP could not only significantly improve the colloidal stability of SLNs and enhance the drug stability in the simulated intestinal fluids, but also intensively increase the intracellular uptake of drugs compared with the other SLNs (p < 0.05). The mechanism of internalization was an active transport involved in clathrin- and caveolae-dependent endocytosis. In vivo, the sCT SLNs prepared with SA and TP exhibited the highest reduction of plasma Ca(2+) level (17.44 ± 3.68%) with a bioavailability of 13.01 ± 3.24%.
Conclusion:
The SLNs formed by SA and TP as the solid lipids may be a promising carrier for oral delivery of peptide drugs.
