Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cytokine levels in serum and cerebrospinal fluid of patients with idiopathic intracranial hypertension.

Brain research·2026
Same author

Successful coil occlusion of intracranial aneurysm in a child with STAT3 hyper IgE syndrome.

Journal of human immunity·2026
Same author

From Conundrums to Consensus: Addressing Gaps in the Management of Leprosy.

The American journal of tropical medicine and hygiene·2026
Same author

Neopterin as a Biomarker of Cellular Immune Response in Renal Allograft Rejection Subtypes: Linking Cytokines and Immune Cells to Improve Diagnostic and Therapeutic Approaches.

Biomedicines·2026
Same author

Patients with congenital ichthyosis show differential activation of IL-23/Th17 pathway among various disease subtypes - A single-centre experience.

Indian journal of dermatology, venereology and leprology·2026
Same author

Exploratory comparative review of wearable-based versus app-based behavioral monitoring tools: evidence synthesis and hypothesis generation.

Expert review of medical devices·2026

Related Experiment Video

Updated: May 17, 2026

Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus
06:28

Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus

Published on: October 1, 2015

Immunofluorescence in dermatology.

Seema Chhabra1, Ranjana Walker Minz, Biman Saikia

  • 1Department of Immunopathology, PGIMER, Chandigarh, India.

Indian Journal of Dermatology, Venereology and Leprology
|October 19, 2012
PubMed
Summary

Direct and indirect immunofluorescence (IF) skin biopsy tests aid dermatologists in diagnosing various skin disorders. These immunofluorescence studies are crucial for identifying bullous diseases, connective tissue disorders, and psoriasis.

Area of Science:

  • Dermatology
  • Immunopathology
  • Histopathology

Background:

  • Direct immunofluorescence (DIF) and indirect immunofluorescence (IIF) are essential diagnostic tools in dermatology.
  • These immunofluorescence (IF) techniques aid in diagnosing various skin disorders, including bullous diseases, connective tissue disorders, vasculitides, lichen planus, and psoriasis.

Purpose of the Study:

  • To review the utility of DIF and IIF in diagnosing skin disorders.
  • To share 30+ years of experience from a tertiary care center in North India.
  • To outline conditions confidently diagnosed and those where IF confirms immune components.

Main Methods:

  • Review of clinical cases and diagnostic procedures over 30 years.
  • Analysis of direct immunofluorescence (DIF) and indirect immunofluorescence (IIF) findings on skin biopsies.

More Related Videos

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research
09:12

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research

Published on: June 10, 2025

Optimization, Design and Avoiding Pitfalls in Manual Multiplex Fluorescent Immunohistochemistry
09:15

Optimization, Design and Avoiding Pitfalls in Manual Multiplex Fluorescent Immunohistochemistry

Published on: July 26, 2019

Related Experiment Videos

Last Updated: May 17, 2026

Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus
06:28

Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus

Published on: October 1, 2015

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research
09:12

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research

Published on: June 10, 2025

Optimization, Design and Avoiding Pitfalls in Manual Multiplex Fluorescent Immunohistochemistry
09:15

Optimization, Design and Avoiding Pitfalls in Manual Multiplex Fluorescent Immunohistochemistry

Published on: July 26, 2019

  • Histological examination and serological data integration.

    • Main Results:

    • Immunofluorescence studies are invaluable supplements to clinical and histological examinations.
    • DIF and IIF assist in diagnosing a wide range of dermatological conditions, including psoriasis and systemic lupus erythematosus.
    • Experience highlights confident diagnoses and confirmation of immune components in skin diseases.

    Conclusions:

    • DIF and IIF are vital diagnostic aids for dermatologists, particularly in academic and tertiary care settings.
    • These immunofluorescence techniques are crucial for delineating and diagnosing various skin disorders and systemic diseases with skin involvement.
    • Proper handling and interpretation of skin biopsy specimens in IF are essential for accurate diagnosis.