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[Expression and significance of Snail and Claudin-3 in non-small cell lung cancer]
1Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, China.
Background And Objective:
Epithelial-mesenchymal transition (EMT) is considered to be one of the major molecular mechanisms inducing tumor invasion and metastasis. The loss of epithelial cell polarity is a hallmark of the EMT process. Epithelial markers such as Claudin are lost in EMT. Snail is a major transcription factor governing EMT. Recent studies about the mechanism of tumor invasion and metastasis has proven that Snail could enhance the ability of many tumors' invasiveness. The aim of this study is to investigate the expression of Snail and Claudin-3 in non-small cell lung cancer (NSCLC) and its metastatic lymph node with tissue microarray technique, and to explore clinical significance of these two molecules expression in NSCLC.
Methods:
Snail and Claudin-3 proteins were detected in cases of 59 adjacent normal lung tissues, 302 cases of primary NSCLC and 57 cases of lymph node metastatic tissues by MaxVision and EnVision method of immunohistochemical staining respectively.
Results:
The expression of Snail in adjacent normal lung tissues, NSCLC primary foci and metastatic lymph node appeared upward tendency (P<0.05), and the expression of Claudin-3 in adjacent normal lung tissues, NSCLC primary foci and metastatic lymph node appeared weakened (P<0.05). Further more, expression 1evel of Snail and Claudin-3 proteins in NSCLC were related to the histological type (P<0.05). The Spearman correlation analysis showed that the expression of Snail and Claudin-3 was negatively correlated (r=-0.178, P=0.002). Kaplan-Meier survival analysis indicated that factors such as the tumor size, histological type, grading, metastasis, TNM staging, and difference expression between two proteins were associated with the postoperative survival rate of NSCLC patients (P<0.05). Multivariate analysis using Cox regression model identified that the tumor size, histological type, grading, metastasis and TNM staging were independent prognostic factors of NSCLC patients (P<0.05).
Conclusions:
Snail and Claudin-3 may play important roles in invasion and metastasis in NSCLC, So might be employed to evaluate the prognosis of NSCLC patients.
Insights
Snail protein expression increases while Claudin-3 decreases in non-small cell lung cancer (NSCLC) and lymph node metastasis. These molecular changes are linked to patient prognosis and could aid in evaluating NSCLC outcomes.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- Epithelial-mesenchymal transition (EMT) drives tumor invasion and metastasis, characterized by loss of epithelial markers like Claudin.
- Snail, a key transcription factor in EMT, enhances tumor invasiveness.
- Understanding Snail and Claudin-3 roles is crucial for NSCLC progression insights.
Purpose of the Study:
- To investigate Snail and Claudin-3 expression in non-small cell lung cancer (NSCLC) and metastatic lymph nodes.
- To explore the clinical significance of Snail and Claudin-3 expression in NSCLC prognosis.
Main Methods:
- Immunohistochemical staining (MaxVision and EnVision) was used to detect Snail and Claudin-3 proteins.
- Tissue samples included adjacent normal lung tissues (59), primary NSCLC (302), and metastatic lymph nodes (57).
Main Results:
- Snail expression showed an upward tendency, while Claudin-3 expression weakened from normal tissues to NSCLC and metastatic lymph nodes (P<0.05).
- Expression levels correlated with NSCLC histological type and were negatively correlated between Snail and Claudin-3 (r=-0.178, P=0.002).
- Tumor size, histological type, grading, metastasis, TNM staging, and differential Snail/Claudin-3 expression were associated with survival.
Conclusions:
- Snail and Claudin-3 play significant roles in NSCLC invasion and metastasis.
- These molecules may serve as valuable biomarkers for evaluating NSCLC patient prognosis.
