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Updated: May 17, 2026
Analysis of Histone Antibody Specificity with Peptide Microarrays
Published on: August 1, 2017
Mimotope identification using phage displayed random peptide libraries against monoclonal antibodies specific to
Nipaporn Tewawong1, Pannamthip Pitaksajjkul, Paron Dekumyoy
1Center of Excellence for Antibody Research (CEAR), Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Abstract:
Random heptapeptide T7 and random 12mer M13 phage libraries were employed to identify mimotopes binding to monoclonal antibodies (MAb) specific to house dust mite. After selection of bound phage by bio-panning and determination of binding specificity, DNA of selected bound phages was amplified, sequenced and aligned for peptide similarity. Eight mimotopes which were partially matched with Der f 15 allergen were predominant. The amino acid regions, 411-429 and 480-503 of Der f 15 allergen, appeared to be the main epitope clusters. Five mimotopes of MAb B2 and one mimotope of MAb B1 matched with Der p 1 and Der f 2 precursor, respectively.
