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[Development and validation of a GC-MS method for determination of nicotine in tobacco]
Doina Spaiuc1, A F Spac, Luminiţa Agoroaei
1Universităţii de Medicină şi Farmacie "Grigore T. Popa"--Iaşi.
Unlabelled:
DETERMINATION FROM TOBA gas chromatographic-mass spectrometric method (GC/MS) for determination of nicotine from tobacco products was developed and validated.
Material And Method:
It was used an Agilent Technologies 7890A Chromatograph equipped with a DB 5 MS column (30 m x 0.25 mm, 0.25 microm) and Agilent Technologies 5975C inert MSD detector. The temperature program began from 100 degrees C and increase with 10 degrees C/min to 190 degrees C than with 20 degrees C/min to 280 degrees C, constant for 5 min, with He as carrier gas (1 ml/min) and MS detection. The temperature of the source was 230 degrees C and the temperature of the quadrupole was 150 degrees C. The parameters of validation were studied according to the international requirements.
Results:
Finally, the method established has following parameters: is linear in the 1.01-201.8 microg/ml range, has the detection limit of 3.6 microg/ml, the quantification limit of 10.8 microg/ml, is precise (RSD < 5%, n = 9) and accurate (mean recovery is 100.28% in 99.08-100.96 microg/ml range).
Conclusion:
The method can be used to determine nicotine from tobacco products, with good results.
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