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Related Experiment Video

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Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach

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Published on: March 12, 2017

Pervasive antisense transcription is evolutionarily conserved in budding yeast.

Aaron J Goodman¹, Evan R Daugharthy, Junhyong Kim

¹Department of Biology, School of Arts and Sciences, University of Pennsylvania, USA.

Molecular Biology and Evolution

|October 20, 2012

Summary

Widespread antisense transcription is common in yeast and evolutionarily conserved. These noncoding RNA transcripts show conserved sequences, suggesting functional roles in gene regulation.

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Area of Science:

Molecular Biology
Genomics
Yeast Genetics

Background:

Antisense transcription, occurring on the opposite DNA strand, is known in various organisms.
Some yeast antisense transcripts are conserved and linked to gene regulation.

Purpose of the Study:

To investigate the prevalence and evolutionary conservation of antisense transcription in budding yeast.
To determine if conserved antisense transcription implies functional significance.

Main Methods:

Genome-wide analysis of antisense transcription in Saccharomyces cerevisiae and S. paradoxus.
Examination of histone modifications associated with antisense transcription.
Comparative analysis of sequence divergence in conserved antisense regions.

Main Results:

Antisense transcription detected from approximately 90% of protein-coding genes.
Antisense transcription is linked to active transcriptional marks (histone modifications).
Genome-wide antisense transcription patterns are conserved between S. cerevisiae and S. paradoxus, including expression and localization.

Conclusions:

Widespread antisense transcription is a conserved feature in yeast, suggesting functional importance.
Conserved antisense regions exhibit reduced sequence divergence, indicating functional constraints.
This study expands the known repertoire of functional antisense transcripts to include nonpolyadenylated forms.