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Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Construction of Cell-based Neurotransmitter Fluorescent Engineered Reporters (CNiFERs) for Optical Detection of Neurotransmitters In Vivo
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A new boronic acid based fluorescent reporter for catechol.

Zhongyu Wu1, Minyong Li, Hao Fang

  • 1Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmacy, Shandong University, 44 West Wenhua Rd., Ji'nan 250012, China.

Bioorganic & Medicinal Chemistry Letters
|October 20, 2012
PubMed
Summary

Researchers developed a new fluorescent sensor, 2-(4-boronophenyl)quinoline-4-carboxylic acid, to detect catechol-containing molecules. This novel reporter shows significant fluorescence changes in aqueous solutions upon binding, aiding in biological detection.

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Published on: August 19, 2013

Area of Science:

  • Analytical Chemistry
  • Organic Chemistry
  • Biochemistry

Background:

  • The catechol structural motif is prevalent in natural products and biologically active compounds.
  • Developing selective fluorescent reporters for catechols is crucial for creating effective chemosensors.
  • Detecting catechols and their derivatives in biological settings presents a significant analytical challenge.

Purpose of the Study:

  • To design and synthesize a novel fluorescent reporter molecule capable of recognizing catechol-containing substances.
  • To investigate the fluorescent properties of the developed reporter in response to catechol binding.
  • To establish the utility of this reporter for detecting catechol derivatives in aqueous environments.

Main Methods:

  • Synthesis of 2-(4-boronophenyl)quinoline-4-carboxylic acid.
  • Spectroscopic analysis (fluorescence spectroscopy) to monitor changes upon interaction with catechol derivatives.
  • Testing the reporter's performance in aqueous solutions.

Main Results:

  • The synthesized compound, 2-(4-boronophenyl)quinoline-4-carboxylic acid, functions as an effective catechol reporter.
  • Significant and measurable changes in fluorescence properties were observed upon the reporter binding to catechol-containing molecules.
  • The reporter demonstrated efficacy in aqueous solutions, indicating potential for biological applications.

Conclusions:

  • 2-(4-boronophenyl)quinoline-4-carboxylic acid is a novel and effective fluorescent reporter for catechols.
  • The observed fluorescence changes provide a basis for developing sensitive chemosensors.
  • This reporter holds promise for the detection of catechol derivatives in biological systems.