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NF-κB-dependent Signaling Pathway

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The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
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Related Experiment Video

Updated: May 17, 2026

NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells

NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells

Published on: January 12, 2020

NF-κB controls Il2 and Csf2 expression during T cell development and activation process.

Yan Li¹, Stephen J Ohms, Chao Sun

¹College of Animal Science & Technology, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China.

Molecular Biology Reports

|October 20, 2012

Summary

This summary is machine-generated.

Immune system aging involves increased inducible genes (Il2, Csf2) and memory T cells. This correlates with higher NF-κB family gene expression, suggesting a mechanism for immune dysregulation during aging.

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Area of Science:

Immunology
Cellular Aging
Molecular Biology

Background:

Immune system aging is linked to dysregulation via unclear mechanisms.
Factors like DNA repair efficiency, transcription factor activity (NF-κB), and cell type shifts influence aging.
Understanding the aging immune system is crucial.

Purpose of the Study:

To investigate the molecular mechanisms underlying immune system aging.
To examine the expression of inducible genes and cell population changes in aging T cells.

Main Methods:

Analysis of inducible gene expression (Il2, Csf2) during T cell activation and aging.
Assessment of circulating CD4(+) T cell population composition.
Measurement of mRNA levels for NF-κB family genes.

Main Results:

Inducible genes Il2 and Csf2 expression increases with T cell activation and aging.
A higher percentage of memory/effector T cells is observed in mature animals.
mRNA levels of NF-κB family genes are significantly elevated in adult mice and activated T cells.

Conclusions:

T cell activation and aging are associated with increased expression of inducible genes Il2 and Csf2.
The aging immune system exhibits a shift towards memory/effector T cells.
Elevated NF-κB family gene expression accompanies these changes, suggesting its role in immune aging.