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Suppressive effect of triadimefon, a triazole fungicide, on spatial learning and reference memory in rats
Jinlei Xi1, Zheqiong Yang, Cheng Zeng
1Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan University, Wuhan, Hubei, China.
Abstract:
Although some central effects of triadimefon, a triazole fungicide, have been reported, its effects on spatial memory have not been examined. In this study, we used the Morris water maze to study the effect of triadimefon on spatial learning and memory in rats. To elucidate the mechanism of this effect, we also measured the retinoic acid concentration in the hippocampus by high-performance liquid chromatography. Our data showed that triadimefon inhibited spatial learning and impaired spatial reference memory, and decreased hippocampal retinoic acid concentration. There is evidence that triadimefon can regulate the metabolism of retinoic acid, which serves a critical function in the development and maintenance of spatial memory. Therefore, we speculate that the reduction in hippocampal retinoic acid concentration induced by triadimefon might be responsible for its suppressive effect on spatial learning and reference memory.
Insights
Triadimefon, a triazole fungicide, impairs spatial learning and memory in rats. This fungicide reduces hippocampal retinoic acid levels, suggesting a link between retinoic acid metabolism and memory function.
Area of Science:
- Neuroscience
- Toxicology
- Biochemistry
Background:
- Triazole fungicides, like triadimefon, are widely used in agriculture.
- Previous studies indicated central nervous system effects of triadimefon.
- The impact of triadimefon on spatial memory and its underlying mechanisms remained uninvestigated.
Purpose of the Study:
- To investigate the effects of triadimefon on spatial learning and memory in a rat model.
- To explore the potential mechanism by examining retinoic acid levels in the hippocampus.
Main Methods:
- Utilized the Morris water maze test to assess spatial learning and memory.
- Quantified hippocampal retinoic acid concentration using high-performance liquid chromatography.
Main Results:
- Triadimefon significantly inhibited spatial learning.
- Impaired spatial reference memory was observed in rats exposed to triadimefon.
- A notable decrease in hippocampal retinoic acid concentration was detected.
Conclusions:
- Triadimefon exposure negatively impacts spatial learning and memory.
- The observed memory deficits may be attributed to triadimefon-induced reduction in hippocampal retinoic acid.
- Triadimefon's effect on retinoic acid metabolism is a potential mechanism for its neurotoxic effects on memory.
