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Published on: June 11, 2020
Ratiometric activatable cell-penetrating peptides provide rapid in vivo readout of thrombin activation
Michael Whitney1, Elamprakash N Savariar, Beth Friedman
1Department of Pharmacology, UCSD School of Medicine, University of California San Diego, La Jolla, USA.
Angewandte Chemie (International Ed. in English)
|October 20, 2012
Abstract:
In real time: thrombin activation in vivo can be imaged in real time with ratiometric activatable cell penetrating peptides (RACPPs). RACPPs are designed to combine 1) dual-emission ratioing, 2) far red to infrared wavelengths for in vivo mammalian imaging, and 3) cleavage-dependent spatial localization. The most advanced RACPP uses norleucine (Nle)-TPRSFL as a linker that increases sensitivity to thrombin by about 90-fold.