Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

D-periodic assemblies of type I procollagen.

A P Mould1, D J Hulmes, D F Holmes

  • 1Department of Medical Biophysics, University of Manchester, England.

Journal of Molecular Biology
|February 5, 1990
PubMed
Summary

Purified chick type I procollagen forms large aggregates above 1.5 mg/ml, including D-periodic assemblies. Intact propeptides on the surface hinder but do not prevent aggregate formation, limiting lateral growth.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Non-oral manifestations in adults with a clinical and molecularly confirmed diagnosis of periodontal Ehlers-Danlos syndrome.

Frontiers in genetics·2023
Same author

Transcriptomic analysis of hepatocellular carcinoma reveals molecular features of disease progression and tumor immune biology.

NPJ precision oncology·2018
Same author

Global curriculum in research literacy for the surgical oncologist.

European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·2017
Same author

Global Curriculum in Research Literacy for the Surgical Oncologist.

Annals of surgical oncology·2017
Same author

Variations in training of surgical oncologists: Proposal for a global curriculum.

European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·2016
Same author

Global curriculum in surgical oncology.

European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·2016

Area of Science:

  • Biochemistry
  • Structural Biology
  • Molecular Biology

Background:

  • Type I procollagen is the precursor to mature type I collagen, a major structural protein.
  • Collagen fibrillogenesis is a complex process involving self-assembly of collagen molecules.
  • The role of propeptide domains in regulating collagen assembly is not fully understood.

Purpose of the Study:

  • To investigate the solubility limit and aggregation behavior of purified chick type I procollagen.
  • To characterize the structure and morphology of procollagen aggregates.
  • To determine the location and influence of propeptide domains on aggregate formation.

Main Methods:

  • Incubation of purified chick type I procollagen in phosphate-buffered saline at 37°C.
  • Separation and characterization of different aggregate types using microscopy and staining techniques.

Related Experiment Videos

  • Immunolabeling to localize propeptide domains within the aggregates.

    • Main Results:

    • Solubility limit of chick type I procollagen is 1-1.5 mg/ml, with aggregate formation at higher concentrations.
    • Identified D-periodic assemblies, narrow filaments, and segment-long-spacing-like aggregates.
    • D-periodic assemblies were ribbon-like (approx. 8 nm thick, up to 1 micron wide) with staining patterns similar to native collagen fibrils.
    • Immunolabeling revealed propeptide domains on the surface, near specific junctions, suggesting they influence assembly.

    Conclusions:

    • Intact propeptide domains of type I procollagen hinder, but do not prevent, D-periodic assembly formation.
    • Propeptide domains on the surface of growing assemblies likely restrict lateral growth and final thickness.
    • These findings provide insights into the molecular mechanisms regulating collagen fibrillogenesis.