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Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
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A peptide photoaffinity probe specific for the active conformation of the Abl tyrosine kinase

Yang Deng1, Brian A Couch, Anthony J Koleske

  • 1Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06520, USA.

Chembiochem : a European Journal of Chemical Biology
|October 20, 2012
PubMed
Abstract

No abstract available in PubMed .

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...