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Purification of a High Molecular Mass Protein in Streptococcus mutans
09:51

Purification of a High Molecular Mass Protein in Streptococcus mutans

Published on: September 14, 2019

Functional amyloid formation by Streptococcus mutans.

M W Oli1, H N Otoo1, P J Crowley1

  • 1University of Florida, Department of Oral Biology, Gainesville, FL, USA.

Microbiology (Reading, England)
|October 20, 2012
PubMed
Summary
This summary is machine-generated.

Streptococcus mutans, a key cause of dental caries, forms biofilms through amyloid proteins. Inhibiting amyloid formation significantly reduces biofilm development in this oral bacterium.

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Area of Science:

  • Microbiology
  • Oral Health
  • Biochemistry

Background:

  • Dental caries is a prevalent infectious disease caused by acidogenic bacteria like Streptococcus mutans.
  • These bacteria form resilient biofilms, producing acids from sugars and tolerating acidic environments.
  • Microbial functional amyloids are increasingly recognized as crucial for biofilm development.

Purpose of the Study:

  • To investigate whether Streptococcus mutans produces functional amyloids.
  • To determine the role of amyloid formation in Streptococcus mutans biofilm development.
  • To identify specific amyloid-forming proteins in Streptococcus mutans.

Main Methods:

  • Amyloid properties of the S. mutans P1 adhesin were assessed using Congo red and Thioflavin T dyes.
  • Amyloid fibers were visualized via transmission electron microscopy.
  • Biofilm formation was evaluated in the presence and absence of amyloid inhibitors and in S. mutans mutants lacking sortase.

Main Results:

  • The S. mutans P1 adhesin was confirmed as an amyloid-forming protein.
  • Amyloid structures were detected in human dental plaque and produced by S. mutans strains.
  • Inhibition of amyloid formation, including by sortase-deficient mutants, significantly impaired S. mutans biofilm formation.

Conclusions:

  • Streptococcus mutans is an amyloid-forming bacterium.
  • Amyloidogenesis is a significant contributor to biofilm formation in Streptococcus mutans.
  • Targeting amyloid formation presents a potential strategy for preventing dental caries.