Effect of controlling blood glucose level on apoptosis in type II diabetes mellitus

Diabetes mellitus (DM) and its complications are complex syndromes representing universal health problems. Egypt ranks ninth among countries with diabetes where the problem is magnified. Researchers have discussed the effect of hyper glycaemia on apoptosis. However, the aims of this study were to assess CD95 positive cells in controlled and uncontrolled type II diabetic patients and to elucidate the effect of controlling blood glucose level on apoptosis. The study was a case control, included controlled type II diabetics and uncontrolled with or without complications) and healthy control groups. Apoptosis was detected by serum DNA fragmentation using ELISA and CD95 positive cells by flow cytometry. There was highly significant increased serum DNA fragmentation and CD95-positive cells in uncontrolled TII DM (complicated and non-complicated) than controlled DM and healthy control groups. In conclusions, poor glycemic control was associated with increased apoptosis.