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Updated: May 17, 2026
08:58
Using R, Seurat, and CellChat to Analyze a Single-Cell Transcriptomics Dataset of Mouse Skin Wound Healing
Published on: August 1, 2025
Human skin transcriptome during superficial cutaneous wound healing
Kristo Nuutila1, Antti Siltanen, Matti Peura
1Institute of Biomedicine, Pharmacology, Biomedicum, University of Helsinki, Helsinki, Finland.
Summary
This study details the genes involved in normal skin reepithelialization after wounding. Understanding these gene patterns offers new insights into epidermal wound healing and potential therapeutic targets.
Area of Science:
- Dermatology
- Molecular Biology
- Genomics
Background:
- Epidermal healing is vital for skin integrity and defense.
- Impaired wound healing increases infection risk and disrupts homeostasis.
Purpose of the Study:
- To elucidate the human transcriptome during normal epidermal wound healing.
- To identify genes and their expression patterns during reepithelialization.
Main Methods:
- Collected split-thickness skin graft donor site wound biopsies over time (0, 3, 7 days).
- Analyzed 25 patient biopsies using genome-wide microarrays.
Main Results:
- Identified genes crucial for normal skin reepithelialization.
- Cataloged genes based on induction or suppression patterns during healing.
- Provided a comprehensive view of the wound healing transcriptome.
Conclusions:
- This study offers the first detailed insight into the human epidermal wound healing transcriptome.
- Novel genes associated with reepithelialization were discovered.
- The findings provide a basis for interpreting experimental wound healing models.
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