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Genetic interactions between protein phosphatases CaPtc2p and CaPph3p in response to genotoxins and rapamycin in

Jinrong Feng1, Yunying Zhao, Yinong Duan

  • 1Department of Pathogen Biology, School of Medicine, Nantong University, Nantong, China.

FEMS Yeast Research
|October 23, 2012
PubMed

Insights

In Candida albicans, CaPtc2p and CaPph3p are not essential for DNA damage recovery. However, their combined absence increases sensitivity to DNA damaging agents and affects filamentation and rapamycin response.

Area of Science:

  • Molecular Biology
  • Mycology
  • Cellular Biology

Background:

  • Protein phosphatases play crucial roles in DNA damage response pathways.
  • In Saccharomyces cerevisiae, ScPtc2p, ScPtc3p, and ScPph3 are involved in dephosphorylating ScRad53p after DNA damage.
  • Understanding homologous protein functions in other fungi like Candida albicans is important for comparative genomics and cellular processes.

Purpose of the Study:

  • To investigate the roles of CaPtc2p and CaPph3p in DNA damage response and other cellular processes in Candida albicans.
  • To determine if CaPtc2p and CaPph3p are involved in CaRad53p dephosphorylation in C. albicans.
  • To explore the potential involvement of these phosphatases in filamentation and target of rapamycin (TOR) signaling.

Main Methods:

  • Gene deletion and mutant analysis in Candida albicans.
  • Sensitivity assays using methylmethane sulfonate (MMS) and hydroxyurea (HU).
  • In vitro filamentation assays and epistasis analysis for rapamycin sensitivity.

Main Results:

  • CaPtc2p and CaPph3p are not required for CaRad53p dephosphorylation after DNA damage in C. albicans.
  • Deletion of CaPPH3 exacerbates the MMS and HU sensitivity of CaPTC2 deletion mutants.
  • CaPPH3 deletion promotes in vitro filamentation, and CaPTC2 mutation is epistatic to CaPPH3 mutation regarding rapamycin sensitivity.

Conclusions:

  • CaPtc2p and CaPph3p have partially redundant roles in DNA damage tolerance in C. albicans.
  • These phosphatases may contribute to regulating filamentation and TOR signaling pathways in this fungal species.

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