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Related Concept Videos

NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
Applications Of NMR In Biology01:25

Applications Of NMR In Biology

Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
The...
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...

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Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

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Published on: September 17, 2017

Microcoils and microsamples in solid-state NMR.

Kazuyuki Takeda1

  • 1Division of Chemistry, Graduate School of Science, Kyoto University, 606-8502 Kyoto, Japan. takezo@kuchem.kyoto-u.ac.jp

Solid State Nuclear Magnetic Resonance
|October 23, 2012
PubMed
Summary
This summary is machine-generated.

Microcoils enhance Nuclear Magnetic Resonance (NMR) signal intensity, especially with smaller coil sizes. Solid-state NMR benefits from microcoil magic-angle spinning (MAS) and strong pulses, despite challenges like the Bloch-Siegert effect.

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MRM Microcoil Performance Calibration and Usage Demonstrated on Medicago truncatula Roots at 22 T
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Area of Science:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Materials Science
  • Physical Chemistry

Background:

  • Microcoils are crucial for enhancing NMR signal intensity.
  • Sample geometry and coil size significantly impact NMR sensitivity.
  • Solid-state NMR presents unique challenges and opportunities with microcoil technology.

Purpose of the Study:

  • To review recent advancements in microcoil technology for NMR.
  • To discuss the influence of geometric factors on NMR signal intensity.
  • To explore microcoil applications in solid-state NMR, including magic-angle spinning (MAS) and strong pulse experiments.

Main Methods:

  • Review of existing literature on microcoil NMR.
  • Analysis of geometric effects on NMR signal intensity.
  • Discussion of microcoil-based magic-angle spinning (MAS) techniques (piggyback, MACS).
  • Examination of strong radiofrequency (RF) pulse applications and associated challenges.

Main Results:

  • Signal intensity increases with decreasing coil size.
  • Diluting small samples with inert matter can improve sensitivity when tiny coils are unavailable.
  • Microcoil MAS enables solid-state NMR of small-volume samples.
  • Strong RF pulses in microcoils introduce challenges such as the Bloch-Siegert effect and transient effects.

Conclusions:

  • Microcoils offer significant advantages for NMR sensitivity and solid-state applications.
  • Careful consideration of geometry and experimental parameters is essential for optimal microcoil NMR performance.
  • Active compensation of pulse transients is a promising approach to overcome limitations in strong RF pulse experiments.