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Related Concept Videos

Nuclear Protein Sorting01:34

Nuclear Protein Sorting

Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
Nuclear Localization Signals and Import01:46

Nuclear Localization Signals and Import

Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of  2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
Nuclear Export01:42

Nuclear Export

The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
Additional Subnuclear Structures02:10

Additional Subnuclear Structures

The eukaryotic nucleus is a double membrane-bound organelle that contains nearly all of the cell’s genetic material in the form of chromosomes. It is rightly called the “brain” of the cell as it shoulders the responsibility of responding to various physiological processes, stress, altered metabolic conditions, and other cellular signals. 
The nucleus contains many membrane-less subnuclear organelles or nuclear bodies, such as nucleoli, Cajal bodies, speckles, paraspeckles, etc. These nuclear...
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...

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Related Experiment Video

Updated: May 17, 2026

Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins
12:47

Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins

Published on: December 27, 2016

NSort/DB: an intranuclear compartment protein database.

Kai Willadsen1, Nurul Mohamad, Mikael Bodén

  • 1School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Australia. willadsen@uq.edu.au

Genomics, Proteomics & Bioinformatics
|October 23, 2012
PubMed
Summary
This summary is machine-generated.

NSort/DB is a new database that maps mouse nuclear proteins to their sub-nuclear compartments. This resource aids in understanding nuclear structures and their protein functions.

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Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing
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Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing

Published on: September 20, 2021

Related Experiment Videos

Last Updated: May 17, 2026

Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins
12:47

Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins

Published on: December 27, 2016

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing
06:00

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing

Published on: September 20, 2021

Area of Science:

  • Cell Biology
  • Proteomics
  • Bioinformatics

Background:

  • Sub-nuclear structures are crucial for nuclear processes.
  • Understanding these compartments requires comprehensive protein association data.
  • Existing data is fragmented, hindering systematic analysis.

Purpose of the Study:

  • To introduce NSort/DB, a database detailing mouse nuclear proteome associations with sub-nuclear compartments.
  • To provide a centralized, high-quality resource for researchers studying nuclear organization.

Main Methods:

  • Compilation of data from large-scale curated datasets and detailed experiments.
  • Annotation of non-exclusive protein associations with nuclear structures like chromatin and promyelocytic leukaemia bodies.
  • Development of a searchable database with a web service API.

Main Results:

  • NSort/DB offers curated annotations for mouse nuclear proteins and their compartment associations.
  • The database includes associations with structures such as chromatin and promyelocytic leukaemia bodies.
  • A user-friendly search interface, web service API, and data download are available online.

Conclusions:

  • NSort/DB facilitates systematic analysis of nuclear protein complements.
  • This resource enhances understanding of the functional roles of intra-nuclear compartments.
  • The database supports research into nuclear processes and organization.