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Related Concept Videos

M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
Positive Regulator Molecules01:45

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Inhibition of CDK Activity02:34

Inhibition of CDK Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...

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Related Experiment Video

Updated: May 17, 2026

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Cyclin-dependent kinase 5 is required for normal cerebellar development.

A Kumazawa1, N Mita, M Hirasawa

  • 1Laboratory for Molecular Brain Science, Department of Life Science and Medical Bioscience, Science and Engineering, Waseda University, Tokyo 162-8480, Japan.

Molecular and Cellular Neurosciences
|October 23, 2012
PubMed
Summary

Cyclin-dependent kinase 5 (Cdk5) is crucial for cerebellar development. Conditional knockout mice showed reduced cerebellum size, impaired neuronal migration, and abnormal dendrite formation in Purkinje cells.

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Last Updated: May 17, 2026

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
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Published on: May 3, 2018

Modeling Human Cerebellar Development In Vitro in 2D Structure
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Utilizing In Vivo Postnatal Electroporation to Study Cerebellar Granule Neuron Morphology and Synapse Development
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Utilizing In Vivo Postnatal Electroporation to Study Cerebellar Granule Neuron Morphology and Synapse Development

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Molecular Biology

Background:

  • Cyclin-dependent kinase 5 (Cdk5) is a key enzyme in neuronal development.
  • Cdk5 activity relies on regulatory subunits p35 or p39, primarily found in neurons.
  • Previous studies showed Cdk5 knockout mice have severe developmental defects.

Purpose of the Study:

  • To investigate the specific role of Cdk5 in cerebellar development.
  • To overcome perinatal lethality issues in Cdk5 knockout mice.

Main Methods:

  • Generation of midbrain-hindbrain-specific Cdk5 conditional knockout (MHB-Cdk5 KO) mice.
  • Histological analysis of cerebellar development in MHB-Cdk5 KO mice.
  • In vitro culture of Cdk5-null Purkinje cells.

Main Results:

  • MHB-Cdk5 KO mice exhibited a significantly reduced cerebellum size.
  • Inward migration of granule cells (GC) was profoundly disturbed.
  • Dendritic development of Purkinje cells (PCs) was abnormal in both KO mice and cultured cells.

Conclusions:

  • Cdk5/p35 signaling is essential for cerebellar development.
  • Cdk5 plays a critical role in neuronal migration of PCs and GCs.
  • Cdk5 is vital for proper dendrite formation in Purkinje cells.