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Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues

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High throughput paper spray mass spectrometry analysis.

Luhou Shen¹, Johnny Zhang, Qian Yang

¹Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA.

Clinica Chimica Acta; International Journal of Clinical Chemistry

|October 23, 2012

Summary

A new paper spray mass spectrometry device enables rapid chemical analysis in complex mixtures. This high-throughput method achieves fast sample analysis with low detection limits for applications like drug monitoring and food safety.

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Area of Science:

Analytical Chemistry
Mass Spectrometry

Background:

Fast chemical analysis is crucial for applications such as therapeutic drug monitoring and food safety.
Complex mixtures require efficient analytical techniques.

Purpose of the Study:

To develop and evaluate a high-throughput device for rapid quantitative analysis.
To assess the performance of paper spray mass spectrometry for complex samples.

Main Methods:

Development of a high-throughput device based on recent paper spray technology.
Quantitative analysis using paper spray mass spectrometry.
Characterization of method performance using spiked bovine blood samples.

Main Results:

Achieved a fast analysis speed of 7 seconds per sample under optimized conditions.
Obtained good limits of quantitation (LOQs) at 1 ng/ml for sunitinib and nicotine in blood.
Demonstrated low limits of detection (LODs) below 1 ng/g for clenbuterol in beef for food screening.

Conclusions:

The developed paper spray mass spectrometry device offers a rapid and sensitive analytical method.
The technology is applicable for both therapeutic drug monitoring and food safety screening.
Achieved high throughput and low detection limits suitable for routine analysis.