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Mechanistic target of rapamycin in common carp: cDNA cloning, characterization, and tissue expression

Jun Jiang1, Lin Feng, Yang Liu

  • 1Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, China.

Gene
|October 23, 2012
PubMed

Insights

Researchers identified the mechanistic target of rapamycin (mTOR) gene in common carp. mTOR gene expression varied across tissues as fish grew, with highest levels in heart and muscle, aiding understanding of juvenile fish development.

Area of Science:

  • Aquaculture
  • Molecular Biology
  • Fish Physiology

Background:

  • The mechanistic target of rapamycin (mTOR) is crucial for cellular growth and organismal development.
  • Understanding mTOR's role in fish is vital for aquaculture and developmental biology.

Purpose of the Study:

  • To identify and characterize the mTOR gene in common carp (Cyprinus carpio).
  • To investigate the tissue-specific expression profile of mTOR mRNA during common carp growth.

Main Methods:

  • cDNA identification and sequencing of common carp mTOR.
  • Bioinformatic analysis to determine protein domains and family.
  • Real-time quantitative PCR (qPCR) to analyze mTOR mRNA abundance in various tissues at different growth stages.

Main Results:

  • A cDNA encoding a 2515-amino acid mTOR protein was identified, showing high similarity to zebrafish counterparts.
  • Bioinformatic analysis confirmed mTOR belongs to the PI-3 kinase family with conserved domains (FAT, FRB, PI3Kc, FATC).
  • mTOR mRNA expression was highest in heart (18-31g) and muscle (31-75g). Expression levels changed dynamically with growth, generally decreasing in liver and heart while increasing in most other tissues between 18-40g, stabilizing thereafter.

Conclusions:

  • The study successfully identified the common carp mTOR gene and elucidated its expression patterns.
  • Findings provide insights into the biological functions of mTOR in common carp development and growth, particularly in juvenile stages.

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