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Updated: May 17, 2026

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
Published on: October 7, 2020
Minimization of nitrous oxide emission from anoxic-oxic biological nitrogen removal process: effect of influent
Zhen Hu1, Jian Zhang, Huijun Xie
1Shandong Provincial Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Jinan, PR China.
Abstract:
Nitrous oxide (N(2)O) is an important greenhouse gas and biological nitrogen removal process of wastewater treatment plant is one of its sources. Mechanisms of N(2)O emissions from anoxic-oxic biological nitrogen removal process were investigated and minimizations of N(2)O emissions were carried out from the aspect of organic carbon supplement, i.e., influent COD/NH4+ ratio (C/N ratio) and feeding strategy. Results showed that during anoxic-oxic biological nitrogen removal process, most of the N(2)O emissions occurred during the oxic phase, and both nitrifier denitrification and aerobic hydroxylamine oxidation pathways were possible mechanisms responsible for N(2)O emissions. N(2)O conversion rate decreased from 6.0% to 1.3% when the influent C/N ratio was increased from 7.5 to 14.5. This was mainly because of decrease in the abundance of Nitrosomonas-like ammonia oxidizing bacteria. Step feeding and external carbon source addition could reduce N(2)O conversion rate by 66.6% and 12.0%, respectively. Both of them were feasible methods for minimizing N(2)O emission from wastewater treatment process. The low N(2)O emission of step feeding was because of its high dissolved oxygen (DO) and low ammonium concentrations during the oxic phase, while the minimization effect of external carbon source addition was ascribed to its high nitrogen removal efficiency.
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