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A multi-center ring trial of allergen analysis using fluorescent multiplex array technology.

Eva M King1, Stephanie Filep, Bryan Smith

  • 1INDOOR Biotechnologies Inc, Charlottesville, VA 22903, USA. eking@inbio.com

Journal of Immunological Methods
|October 23, 2012
PubMed
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This summary is machine-generated.

This study shows fluorescent multiplex array technology provides reproducible indoor allergen measurements across multiple labs. This consistency is vital for accurate asthma and allergy exposure assessments.

Area of Science:

  • Environmental Science
  • Immunology
  • Analytical Chemistry

Background:

  • Accurate allergen quantification is crucial for asthma and occupational allergy research.
  • Reproducibility of allergen assays ensures reliable exposure assessment.
  • This study assesses a fluorescent multiplex array for simultaneous indoor allergen measurement.

Purpose of the Study:

  • To evaluate the intra- and inter-laboratory reproducibility of an 8-plex fluorescent multiplex array.
  • To determine the variability in measuring eight indoor allergens using this technology.
  • To assess the suitability of multiplex arrays for standardized allergen testing.

Main Methods:

  • A multi-center study involving nine laboratories in the US and Europe.
  • Analysis of 151 dust extract samples using an 8-plex array for common indoor allergens.

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  • Calculation of agreement using concordance correlation coefficient (CCC) and coefficient of variance (CV%).

    • Main Results:

    • Over 32,000 allergen measurements demonstrated high reproducibility within (94% CCC ≥ 0.90) and between laboratories (correlations ~0.95).
    • Intra-laboratory results showed 80% within a 10% CV, with inter-laboratory CVs ranging from 17.6% to 26.6%.
    • The array accurately quantified a wide range of allergen levels, from below detection limits to over 6800 ng/ml.

    Conclusions:

    • Fluorescent multiplex array technology meets reproducible performance criteria within and between laboratories.
    • This technology offers standardized and consistent allergen measurements.
    • Multiplex arrays can streamline environmental exposure assessments for allergic diseases.