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Related Concept Videos

Bone Marrow Sampling and Transplants01:22

Bone Marrow Sampling and Transplants

Bone marrow transplant is a potential cure for several diseases, including cancer and specific genetic disorders. Notably, this procedure is applicable for patients suffering from aplastic anemia, certain types of leukemia, severe combined immunodeficiency disease (SCID), Hodgkin's disease, non-Hodgkin's lymphoma, multiple myeloma, thalassemia, sickle-cell disease, and certain cancers.
The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy the...

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Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia
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[Bone marrow biopsy: processing and use of molecular techniques].

L Quintanilla-Martinez1, M Tinguely, I Bonzheim

  • 1Institut für Pathologie und Neuropathologie, Universitätsklinikum Tübingen, Liebermeisterstr 8, 72076 Tübingen, Deutschland. leticia.quintanilla-fend@med.uni-tuebingen.de

Der Pathologe
|October 23, 2012
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Summary

Optimizing bone marrow biopsy processing is crucial for accurate diagnostic pathology. Careful workflow planning balances morphology preservation with molecular analysis for hematological neoplasms.

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Area of Science:

  • Diagnostic Pathology
  • Molecular Diagnostics
  • Hematopathology

Context:

  • Advancements in immunohistochemical and molecular techniques impact bone marrow trephine biopsy evaluation.
  • Balancing morphological integrity, antigen/nucleic acid preservation, and rapid turnaround times is essential.
  • Standardization of bone marrow processing workflows is needed.

Purpose:

  • To review technical aspects of bone marrow biopsy processing.
  • To discuss the application of diagnostic molecular techniques in trephine biopsies.
  • To highlight challenges in molecular studies for hematological neoplasms.

Summary:

  • Formalin fixation and EDTA decalcification offer a compromise for preserving morphology and enabling molecular analysis.
  • Molecular studies on trephine biopsies are indicated for clonality determination and mutation detection in specific cases.
  • Stringent quality control and awareness of technical pitfalls are vital for accurate molecular diagnostics, especially for lymphoid malignancies.

Impact:

  • Facilitates informed decisions on bone marrow biopsy processing workflows.
  • Enhances the utility of trephine biopsies for molecular diagnostics in hematological neoplasms.
  • Improves diagnostic accuracy and patient management for hematological malignancies.