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Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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Related Experiment Video

Updated: May 17, 2026

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
09:21

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy

Published on: July 20, 2019

Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy.

Jeffrey D Martell1, Thomas J Deerinck, Yasemin Sancak

  • 1Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

Nature Biotechnology
|October 23, 2012
PubMed
Summary
This summary is machine-generated.

We developed APEX, a new genetic tag for electron microscopy (EM) that works in all cell parts without light. This APEX tag enables clear imaging of cellular structures and proteins, simplifying EM analysis.

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Area of Science:

  • Cell Biology
  • Microscopy Techniques
  • Biochemistry

Background:

  • Electron microscopy (EM) is crucial for nanometer-resolution cellular imaging.
  • Current genetic tags for EM have limitations, including compartment specificity, light dependency, and usability issues.

Purpose of the Study:

  • To develop a novel, versatile genetic tag for electron microscopy (EM).
  • To enable high-resolution EM imaging across all cellular compartments without light activation.

Main Methods:

  • Engineered APEX, a genetically encodable 28-kDa peroxidase tag.
  • Tested APEX's activity and stability under EM fixation conditions.
  • Fused APEX to target proteins, including the mitochondrial calcium uniporter (MCU), for localization studies.

Main Results:

  • APEX functions effectively in all cellular compartments and withstands EM fixation, ensuring excellent ultrastructural preservation.
  • Demonstrated high-resolution EM imaging of organelles and proteins using APEX with a simple labeling protocol.
  • APEX fusions to MCU indicated both termini face the mitochondrial matrix, resolving topology disputes.

Conclusions:

  • APEX is a robust and universally applicable genetic tag for electron microscopy.
  • Facilitates straightforward, light-independent EM imaging of any cellular protein, irrespective of specimen size or thickness.