Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Probiotics

Probiotics

Probiotics are live, non-pathogenic microorganisms that confer health benefits by modulating the gut microbiota. The human gastrointestinal tract harbors a complex microbial ecosystem, and the balance of this microbiota is crucial for digestive and systemic health. Among the most extensively studied and utilized probiotics are species formerly classified within the genera Lactobacillus and Bifidobacterium. These organisms not only naturally colonize the human gut but are also consumed through...

Microbiota of the Stomach and Small Intestine

Microbiota of the Stomach and Small Intestine

The human gastrointestinal (GI) tract is characterized by distinct physicochemical conditions that shape its microbial communities. Among these, the stomach presents a particularly challenging environment for microbial colonization due to its highly acidic pH, ranging from 1 to 3. This extreme acidity effectively limits microbial density. However, certain acid-tolerant microorganisms are capable of surviving in this niche. Notably, Helicobacter pylori can colonize the gastric mucosa,...

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

Bacterial Flora of the Large Intestine

Bacterial Flora of the Large Intestine

The gut microbiome is formed by a vast and diverse community of bacteria that colonizes our large intestine. These bacteria start residing in the gut from birth and continue diversifying throughout life, influenced by factors such as diet, lifestyle, and stress. The gut bacterial community also includes bacteria from food and those that enter the colon through the anus.
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.

Inducible Operons: lac Operon

Inducible Operons: lac Operon

The lac operon in Escherichia coli is a model for understanding inducible gene regulation and metabolic flexibility. It integrates local control by lactose and global regulation through catabolite repression, enabling E. coli to preferentially metabolize glucose when available and switch to lactose utilization when glucose is scarce.Structure and Function of the lac OperonThe lac operon contains three structural genes: lacZ (β-galactosidase), lacY (lactose permease), and lacA (thiogalactoside...

Microbiota of the Large Intestine

Microbiota of the Large Intestine

The large intestine hosts the most densely populated microbial ecosystem in the human body. This complex community primarily consists of anaerobic bacteria, with Bacillota (formerly Firmicutes) and Bacteroidota (formerly Bacteroidetes) as the predominant groups. The distribution of these microbes varies along different sections of the large intestine, influenced by local environmental factors such as oxygen availability and nutrient composition.The cecum, located at the beginning of the large...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Luminescent heparin-functionalized carbon dots with potential applications in nanoparticle-protein interactions and cell imaging.

Scientific reports·2026

Same author

Marine-Derived Dual BTK-FGFR Inhibitors: Pioneering a New Era of Precision Oncology Therapeutics.

Anti-cancer agents in medicinal chemistry·2026

Same author

Deciphering the multi-organ anti-fibrotic mechanisms of pirfenidone and nintedanib via network pharmacology.

Personalized medicine·2026

Same author

Novel class of population mean estimators based on robust regression methods.

Scientific reports·2026

Same author

Unraveling the Multifunctional and Translational Paradigm of Nanoparticulate Systems against Colorectal Cancer.

ACS applied bio materials·2026

Same author

Quality-by-design-based fabrication of betaxolol hydrochloride-loaded nano-ocular carrier via ternary phase mapping of solid/liquid lipids and BBD modeling for enhanced transcorneal flux and IOP-lowering effect.

AAPS PharmSciTech·2026

Search research articles

Related Experiment Video

Updated: May 17, 2026

A Novel Method for the Culture and Polarized Stimulation of Human Intestinal Mucosa Explants

A Novel Method for the Culture and Polarized Stimulation of Human Intestinal Mucosa Explants

Published on: May 1, 2013

Lactobacillus acidophilus upregulates intestinal NHE3 expression and function.

Varsha Singh¹, Geetu Raheja, Alip Borthakur

¹Section of Digestive Diseases & Nutrition, Dept. of Medicine, University of Illinois at Chicago, Chicago, IL, USA.

American Journal of Physiology. Gastrointestinal and Liver Physiology

|October 23, 2012

Summary

Lactobacillus acidophilus (LA) enhances intestinal electrolyte absorption by increasing Na+/H+ exchanger 3 (NHE3) expression and activity. This mechanism may contribute to the antidiarrheal effects of probiotics.

More Related Videos

Breast Milk Enhances Growth of Enteroids: An Ex Vivo Model of Cell Proliferation

Breast Milk Enhances Growth of Enteroids: An Ex Vivo Model of Cell Proliferation

Published on: February 15, 2018

Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

Published on: November 15, 2019

Related Experiment Videos

Last Updated: May 17, 2026

A Novel Method for the Culture and Polarized Stimulation of Human Intestinal Mucosa Explants

A Novel Method for the Culture and Polarized Stimulation of Human Intestinal Mucosa Explants

Published on: May 1, 2013

Breast Milk Enhances Growth of Enteroids: An Ex Vivo Model of Cell Proliferation

Breast Milk Enhances Growth of Enteroids: An Ex Vivo Model of Cell Proliferation

Published on: February 15, 2018

Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

Published on: November 15, 2019

Area of Science:

Gastroenterology
Molecular Biology
Microbiology

Background:

Electroneutral NaCl absorption in the human ileum and colon relies on coupled Na+/H+ (NHE3) and Cl-/HCO3- (DRA) exchangers.
Disturbances in these exchangers are linked to diarrheal conditions.
Probiotics, like Lactobacillus, show promise in managing gastrointestinal disorders, but their molecular antidiarrheal mechanisms require elucidation.

Purpose of the Study:

To investigate the effects of Lactobacillus acidophilus (LA) on the function and expression of the Na+/H+ exchanger 3 (NHE3).
To determine the molecular mechanisms underlying LA's influence on NHE3.

Main Methods:

Caco2 and SK-CO15 cell lines were treated with LA or its conditioned supernatant (CS).
Na+/H+ exchange activity, NHE3 mRNA, and protein levels were assessed.
NHE3 promoter activity was measured.
Mice were gavaged with live LA, and intestinal tissues were analyzed for NHE3 expression.

Main Results:

LA and LA-CS significantly increased Na+/H+ exchange activity, NHE3 mRNA, and protein levels in intestinal cell lines.
LA treatment enhanced NHE3 promoter activity, indicating transcriptional regulation.
In vivo, LA administration increased NHE3 mRNA and protein expression in mouse ileum and colon.

Conclusions:

Lactobacillus acidophilus upregulates the expression and function of NHE3 in intestinal cells and in vivo.
LA-induced increase in NHE3 may enhance intestinal electrolyte absorption.
This mechanism could underlie the antidiarrheal properties of Lactobacillus acidophilus.