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Updated: May 17, 2026

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates

Published on: February 27, 2016

Sirtuin catalysis and regulation.

Jessica L Feldman¹, Kristin E Dittenhafer-Reed, John M Denu

¹Department of Biomolecular Chemistry and the Wisconsin Institute for Discovery, University of Wisconsin, Madison, Wisconsin 53715, USA. jmdenu@wisc.edu

The Journal of Biological Chemistry

|October 23, 2012

Summary

This summary is machine-generated.

Sirtuins, NAD(+)-dependent enzymes, regulate key cellular processes and are linked to health span and disease. This review covers their chemistry, targets, regulation, and the development of small-molecule drugs.

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Last Updated: May 17, 2026

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates

Published on: February 27, 2016

Area of Science:

Biochemistry and Molecular Biology
Cellular Biology
Pharmacology

Background:

Sirtuins are NAD(+)-dependent enzymes regulating transcription, metabolism, and stress response.
These enzymes are implicated in aging, caloric restriction pathways, and various human diseases.
Therapeutic targeting of sirtuins is of significant interest due to their health implications.

Purpose of the Study:

To review the current understanding of sirtuin enzyme catalysis and biological functions.
To summarize the endogenous mechanisms regulating sirtuin activity.
To discuss the development of small-molecule compounds that modulate sirtuin activity.

Main Methods:

Literature review of sirtuin research.
Analysis of biochemical and cellular studies on sirtuin function.
Overview of medicinal chemistry efforts for sirtuin modulator discovery.

Main Results:

Sirtuins catalyze deacetylation and other deacylation reactions crucial for cellular homeostasis.
Endogenous factors and post-translational modifications dynamically control sirtuin activity.
Small-molecule modulators are being developed to target specific sirtuins for therapeutic benefit.

Conclusions:

Sirtuins are critical regulators of cellular processes with therapeutic potential.
A deeper understanding of sirtuin chemistry and regulation is essential for drug development.
Targeted modulation of sirtuin activity holds promise for treating diseases associated with aging and metabolic dysfunction.