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Related Concept Videos

Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
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Scanning-probe Single-electron Capacitance Spectroscopy
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Published on: July 30, 2013

The scanning ion conductance microscope for cellular physiology.

Max J Lab1, Anamika Bhargava, Peter T Wright

  • 1Imperial College London, National Heart and Lung Institute, Imperial Centre for Experimental and Translational Medicine, London, United Kingdom.

American Journal of Physiology. Heart and Circulatory Physiology
|October 23, 2012
PubMed
Summary
This summary is machine-generated.

Scanning ion conductance microscopy (SICM) offers high-resolution imaging of live biological samples without physical contact. This advanced technique provides topographical data and enables functional studies like ion channel recording.

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Area of Science:

  • Biophysics
  • Nanotechnology
  • Microscopy

Background:

  • Traditional microscopy methods face limitations in resolving biological samples due to light diffraction.
  • Existing high-resolution techniques like atomic force microscopy and scanning electron microscopy are often unsuitable for live biological specimens.
  • There is a need for non-optical imaging methods that combine high resolution with functional analysis of biological systems.

Purpose of the Study:

  • To introduce Scanning Ion Conductance Microscopy (SICM) as a novel technique for high-resolution imaging of biological samples.
  • To highlight SICM's capability to bridge the resolution gap between existing microscopy methods and functional analysis.
  • To demonstrate SICM's versatility for various applications in biological research, including live-cell imaging and functional studies.

Main Methods:

  • Utilizes a nanopipette attached to a three-axis piezo-actuator to scan the sample surface.
  • Measures ion current between the pipette tip and the sample, employing a feedback control system to maintain a constant distance.
  • Records pipette movement to generate a three-dimensional topographical image of the sample without physical contact.

Main Results:

  • SICM provides high-resolution topographical imaging of live biological samples, overcoming light diffraction limits.
  • The nanopipette probe facilitates additional functionalities, such as cell membrane approach and sealing for ion channel recording.
  • SICM can be integrated with optical and fluorescent microscopy for combined structural and functional analysis.
  • The technique allows for precise mechanical force measurements and controlled pressure application on living cells and tissues over extended periods without compromising viability.

Conclusions:

  • Scanning Ion Conductance Microscopy (SICM) is a powerful, multifunctional tool for high-resolution imaging and functional analysis of biological samples.
  • SICM offers a non-invasive alternative to conventional microscopy, particularly for studying live cells and tissues.
  • The continuous development of SICM promises expanded capabilities and applications in future biological and nanotechnological research.