Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

B Cell Activation and Differentiation

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Jump-starting the T cell response in established tumors.

bioRxiv : the preprint server for biology·2026

Same author

Dysregulation of anti-Ro60 B cell autoreactivity in systemic lupus erythematosus.

bioRxiv : the preprint server for biology·2026

Same author

Six-Month Outcomes in Children With COVID-19 or Multisystem Inflammatory Syndrome in Children.

Hospital pediatrics·2026

Same author

Knockdown of the long isoform of the prolactin receptor selectively targets pathogenic immune cells in systemic lupus erythematosus and averts glomerular pathology.

bioRxiv : the preprint server for biology·2026

Same author

Human anti-glycan reactivity emerges from B cells utilizing private gene rearrangements that are affinity maturated in germinal centers.

Immunity·2026

Same author

Chimeric antigen receptor T cells against the IGHV4-34 B cell receptor specifically eliminate neoplastic and autoimmune B cells.

Science translational medicine·2026

Search research articles

Related Experiment Video

Updated: May 17, 2026

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Published on: February 16, 2017

Advances in human B cell phenotypic profiling.

Denise A Kaminski¹, Chungwen Wei, Yu Qian

¹Division of Allergy, Immunology, and Rheumatology, Department of Medicine, University of Rochester Rochester, NY, USA.

Frontiers in Immunology

|October 23, 2012

Summary

This summary is machine-generated.

Human immunology research needs standardized metrics for large-scale studies. This review highlights advances in B cell phenotyping and bioinformatics, enabling B cell research to contribute to "Big Biology" and disease understanding.

Keywords:

B lymphocyte autoimmunity data clustering data management flow cytometry human

More Related Videos

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood

Published on: February 8, 2016

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity

Published on: December 14, 2016

Related Experiment Videos

Last Updated: May 17, 2026

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Published on: February 16, 2017

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood

Published on: February 8, 2016

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity

Published on: December 14, 2016

Area of Science:

Immunology
Computational Biology
Human Health

Background:

Advancing disease understanding and treatment requires a greater focus on large-scale human studies ("Big Biology").
Standardized immune status metrics are crucial for "Big Biology" initiatives, exemplified by T lymphocyte studies in HIV cohorts.
B lymphocytes play significant roles in infection, immunodeficiency, and autoimmunity but are underrepresented in human "Big Biology" research.

Purpose of the Study:

To review progress in human B cell phenotyping, analysis, and bioinformatics tools.
To provide valuable resources for the B cell research community to engage in "Big Biology" efforts.
To facilitate the integration of B cell research into large-scale human health studies.

Main Methods:

Review of current advancements in human B cell phenotyping techniques.
Analysis of existing bioinformatics tools applicable to B cell data.
Synthesis of resources relevant to B cell research in large-scale studies.

Main Results:

Significant progress has been made in human B cell phenotyping methodologies.
A growing suite of bioinformatics tools is available for B cell data analysis.
These advancements provide a foundation for incorporating B cells into "Big Biology" research.

Conclusions:

Standardized B cell metrics and advanced bioinformatics are essential for "Big Biology".
The reviewed resources will empower B cell researchers to contribute to understanding complex diseases.
Integrating B cell analysis into large-scale human studies will enhance disease research and treatment development.