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Updated: May 17, 2026

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

Spectrally resolved chemiluminescent probes for sensitive multiplex molecular quantification.

Kenneth A Browne¹, Dimitri D Deheyn, Richard C Brown

¹Gen-Probe Incorporated, 10210 Genetic Center Drive, San Diego, California 92121, United States. ken.browne@hologic.com

Analytical Chemistry

|October 24, 2012

Summary

Chemically initiated luminophores offer high sensitivity but are hard to distinguish. New hybrid probes enable multiplex detection of analytes like microbial nucleic acids without amplification.

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Area of Science:

Biochemistry
Analytical Chemistry
Molecular Probes

Background:

Fluorescent luminophores are common for analyte detection but distinguishing multiple chemically initiated luminophores is challenging.
Existing methods for multiplexing chemically initiated luminophores are limited, hindering their broader application.

Purpose of the Study:

To develop a new class of hybrid luminophore probes for sensitive, multiplexed detection.
To enable rapid, rational design of spectrally resolvable, chemically initiated probes.

Main Methods:

Developed hybrid luminophore probes with covalently conjugated fluorophores and an acridinium ester chemiluminophore.
Utilized energy transfer (ET) for light emission at distinct wavelengths and intensities.
Performed time-resolved spectrographic and luminescence characterizations.

Main Results:

Demonstrated that ET is not dependent on spectral overlap, suggesting a non-Förster resonance mechanism.
Showcased the probes' ability for sensitive, multiplex quantification of nucleic acid sequences.
Achieved detection of environmentally relevant microbes without prior enzymatic amplification.

Conclusions:

The novel hybrid luminophore probes facilitate multiplexed detection of analytes.
This approach offers a sensitive and efficient method for quantifying microbial nucleic acids.
The findings advance the development of chemically initiated probes for various applications.