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Related Experiment Video

Updated: May 17, 2026

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

LC-MS systems for quantitative bioanalysis.

William D van Dongen¹, Wilfried M A Niessen

¹TNO Triskelion, Utrechtseweg 48, 3704 AV Zeist, The Netherlands. wdvandongen@gmail.com

|October 24, 2012

Summary

Liquid chromatography-mass spectrometry (LC-MS) is a leading technique for small-molecule drug analysis and is emerging for biopharmaceuticals. Both triple quadrupole and high-resolution LC-MS instruments offer unique advantages for bioanalysis.

Area of Science:

Analytical Chemistry
Pharmacology
Biotechnology

Background:

Liquid chromatography-mass spectrometry (LC-MS) is the preferred method for small-molecule drug bioanalysis (<800 Da).
LC-MS is increasingly used as an alternative to ligand-binding assays for biopharmaceutical analysis.
Triple quadrupole MS offers high selectivity and sensitivity, while high-resolution MS provides simultaneous quantitative and qualitative analysis.

Purpose of the Study:

To discuss current trends in bioanalytical LC-MS until September 2012.
To provide an overview of commercial LC-MS instruments for drug bioanalysis.
To compare triple quadrupole and high-resolution MS for small-molecule and biopharmaceutical applications.

Main Methods:

Review of current literature and commercial instrumentation in bioanalytical LC-MS.

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Focus on triple quadrupole and high-resolution mass spectrometry techniques.

Application of LC-MS for both small-molecule and biopharmaceutical drug analysis.

Main Results:

LC-MS is a versatile technique for both small molecules and biopharmaceuticals.
Triple quadrupole MS remains the standard for sensitivity and selectivity.
High-resolution MS is gaining traction for its simultaneous quantitative and qualitative capabilities.
Various commercial instruments are available for both MS types.

Conclusions:

LC-MS is a critical tool in modern drug bioanalysis.
The choice between triple quadrupole and high-resolution MS depends on specific analytical needs.
Advancements in LC-MS technology continue to enhance drug development and monitoring.