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Related Concept Videos

Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Types Of Column Chromatography01:29

Types Of Column Chromatography

The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
Ion Exchange01:17

Ion Exchange

Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or basic...

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Detection of Viruses from Bioaerosols Using Anion Exchange Resin
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Published on: August 22, 2018

Monolithic ion exchange chromatographic methods for virus purification.

Hanna M Oksanen1, Ausra Domanska, Dennis H Bamford

  • 1Institute of Biotechnology and Department of Biosciences, University of Helsinki, Viikinkaari 5, 00014 Helsinki, Finland. hanna.oksanen@helsinki.fi

Virology
|October 24, 2012
PubMed
Summary

This study presents a novel ion exchange chromatography method using large pore size monolithic anion exchangers for ultrapure virus particle purification. This scalable technique effectively purifies bacteriophages, yielding infectious and homogenous particles suitable for various analyses.

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Area of Science:

  • Biochemistry
  • Virology
  • Chromatography

Background:

  • Virus purification is crucial for detailed virological, biochemical, and structural analyses.
  • Traditional methods can be time-consuming and may compromise virus integrity.
  • Development of efficient and scalable purification techniques is essential for advancing virus research.

Purpose of the Study:

  • To develop and validate a novel ion exchange chromatographic purification method for high-quality virus particles.
  • To assess the efficacy of large pore size monolithic anion exchangers for bacteriophage purification.
  • To evaluate the scalability and applicability of the method for different virus types.

Main Methods:

  • Utilized large pore size monolithic anion exchangers (quaternary amine and diethylaminoethyl) for ion exchange chromatography.
  • Applied the method to purify icosahedral bacteriophage PRD1 and filamentous bacteriophage phi05_2302.
  • Assessed virus particle purity, infectivity, homogeneity, and yield post-purification.

Main Results:

  • Achieved ultrapure virus particles with retained infectivity and high specific infectivity.
  • Obtained yields of infectious particles up to 80% for bacteriophage PRD1.
  • Recovered purified virus particles at high concentrations (approximately 5 mg/ml).
  • Demonstrated successful purification of filamentous bacteriophage phi05_2302, indicating broad applicability.

Conclusions:

  • Monolithic ion exchange chromatography offers a powerful and scalable method for preparing ultrapure virus particles.
  • The developed technique preserves virus infectivity and yields homogenous particles suitable for extensive research.
  • This method is easily scalable and can be integrated with other purification strategies for enhanced efficiency.