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Related Concept Videos

PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a rapamycin-insensitive companion...
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Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
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Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors
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Published on: March 17, 2014

PI3K-PKB hyperactivation augments human plasmacytoid dendritic cell development and function.

Lianne van de Laar1, Aniek van den Bosch, André Boonstra

  • 1Departments of Gastroenterology and Hepatology, Erasmus MC-University Medical Center, Rotterdam, The Netherlands.

Blood
|October 24, 2012
PubMed
Summary
This summary is machine-generated.

The phosphatidylinositol 3-kinase (PI3K)-protein kinase B (PKB)-mammalian target of rapamycin (mTOR) pathway is crucial for plasmacytoid dendritic cell (pDC) development, survival, and function. Enhancing this signaling boosts pDC numbers and activity for immunotherapy.

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Last Updated: May 17, 2026

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Assessment of Human Natural Killer Cell Events Driven by FcγRIIIa Engagement in the Presence of Therapeutic Antibodies

Published on: May 22, 2020

Area of Science:

  • Immunology
  • Cell Biology
  • Signaling Pathways

Background:

  • Plasmacytoid dendritic cells (pDCs) are key players in immunotherapy, but methods to control their development and function are limited.
  • The PI3K-PKB-mTOR signaling axis is a critical regulator of cellular processes, but its role in pDCs is not well understood.

Purpose of the Study:

  • To investigate the role of the PI3K-PKB-mTOR pathway in human pDC development, survival, and function.
  • To explore the potential of manipulating this pathway for therapeutic applications in immunotherapy.

Main Methods:

  • In vitro generation of pDCs from human cord blood progenitors.
  • Pharmacologic inhibition or activation of PI3K, PKB, and mTOR signaling components.
  • Analysis of pDC numbers, survival, phenotype (MHC class II, costimulatory molecules), and cytokine production (IFN-α, TNF-α).
  • Assessment of signaling pathway activation (IRF7, NF-κB) and PTEN activity.
  • Evaluation of pDCs from chronic hepatitis B patients.

Main Results:

  • PI3K-PKB-mTOR inhibition reduced pDC generation and survival; pathway activity correlated with pDC abundance.
  • Constitutive PKB activation or PTEN inhibition increased pDC numbers in vitro and in vivo.
  • Enhanced PI3K-PKB-mTOR signaling boosted pDC expression of MHC class II, costimulatory molecules, and production of IFN-α and TNF-α via IRF7 and NF-κB.
  • pDCs from chronic hepatitis B patients showed impaired PI3K-PKB-mTOR activity.

Conclusions:

  • Intact PI3K-PKB-mTOR signaling is essential for human pDC development, survival, and function.
  • Hyperactivation of PI3K-PKB signaling can promote pDC development and enhance their functionality.
  • Targeting the PI3K-PKB-mTOR pathway offers a promising strategy to improve pDC-based immunotherapies.