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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
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Published on: September 29, 2012

Comparing proteomics and RISC immunoprecipitations to identify targets of Epstein-Barr viral miRNAs

Malika Kuzembayeva1, Ya-Fang Chiu, Bill Sugden

  • 1McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, Wisconsin, United States of America.

Plos One
|October 24, 2012
PubMed

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Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...