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Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6
Published on: July 29, 2021
A highly fluorescent pH sensing membrane for the alkaline pH range incorporating a BODIPY dye
Mandy Hecht1, Werner Kraus, Knut Rurack
1Div. 1.9 Sensor Materials, BAM Federal Institute for Materials Research and Testing, Richard-Willstätter-Str. 11, D-12489 Berlin, Germany.
Abstract:
A robust and re-usable dipstick-type fluorescent pH sensor for the alkaline pH range was developed by embedding a brightly fluorescent boron-dipyrromethene (BODIPY) dye bearing an acidic phenol moiety into a polyurethane matrix immobilized on a 3D epoxy-functionalized polymer support. The sensor strip has a dynamic working range of pH 10.0-13.1, i.e., operates in strongly basic media where pH glass electrodes can suffer from alkaline errors, and tolerates a high electrolyte background such as simulated seawater and sewage. This work describes the preparation of the sensing material and provides insight into the features that a hydrogel sensing membrane can bestow on an embedded pH-responsive dye by means of optical spectroscopic investigations.
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