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Updated: May 17, 2026

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
Published on: March 13, 2014
Binuclear manganese (III) complexes as electron donors in D1/D2/cytochrome b559 preparations isolated from spinach
S I Allakhverdiev1, M S Karacan, G Somer
1Institute of Soil Science and Photosynthesis, RAS, Puschino, Moscow Region 142292 Russia; Department of Chemistry, University of Gazi, Ankara, 06500, Turkey; Department of Chemistry, University of Poona, 411007, India.
Abstract:
The capability of different manganese complexes to act as PS II electron donors in D1/D2/cytochrome b559 complexes has been analyzed by measuring actinic light-induced absorption changes at 680 nm (650 nm) and 340 nm, reflecting the photoaccumulation of Pheophytin⁻ (Pheo⁻) and the reduction of NADP⁺, respectively. The data obtained revealed: a) the donor capacity of synthetic binuclear Mn (III)₂complexes containing aromatic ligands significantly exceeds that for MnCl₂ in both cases, i.e. Pheo⁻ photoaccumulation and NADP⁺ reduction; b) manganese complexes can serve as suitable electron donors for light-induced NADP⁺ reduction catalyzed by D1/D2/cytochrome b559 complexes and ferredoxin plus ferredoxin-NADP⁺ reductase under anaerobic conditions and c) the specific turnover rate of the system leading to NADP⁺ reduction is extremely small. The implications of these findings are briefly discussed.
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