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Embryotoxic evaluation of bis(tri-n-butyltin)oxide (TBTO) in mice
S Baroncelli1, D Karrer, P G Turillazzi
1Laboratorio di Tossicologia Comparata ed Ecotossicologia, Istituto Superiore di Sanità, Rome, Italy.
Abstract:
Pregnant mice were treated on days 6-15 of gestation with 5, 20 and 40 mg/kg/d bis(tri-n-butyltin)oxide (TBTO), and sacrificed on gestational day 17. At the highest dose TBTO caused a significant reduction of maternal body weight gain and also proved to be highly embryotoxic. Necropsy showed a dose-related decrease in spleen weight while a dose-dependent increase in placental weight was observed.
Insights
Bis(tri-n-butyltin)oxide (TBTO) exposure during pregnancy in mice caused significant maternal weight reduction and embryotoxicity. Organ weights were affected, with spleen weight decreasing and placental weight increasing dose-dependently.
Area of Science:
- Toxicology
- Developmental Biology
- Reproductive Toxicology
Background:
- Bis(tri-n-butyltin)oxide (TBTO) is an organotin compound with known toxicological properties.
- Understanding the developmental toxicity of environmental contaminants is crucial for public health.
Purpose of the Study:
- To investigate the embryotoxic and developmental effects of bis(tri-n-butyltin)oxide (TBTO) in pregnant mice.
- To assess the impact of TBTO on maternal and fetal health during gestation.
Main Methods:
- Pregnant mice were administered varying doses of bis(tri-n-butyltin)oxide (TBTO) during critical gestational periods (days 6-15).
- Maternal body weight gain and organ weights (spleen, placenta) were measured.
- Embryotoxicity was assessed at gestational day 17.
Main Results:
- A significant reduction in maternal body weight gain was observed at the highest TBTO dose.
- Bis(tri-n-butyltin)oxide (TBTO) demonstrated high embryotoxicity.
- A dose-related decrease in spleen weight and a dose-dependent increase in placental weight were noted.
Conclusions:
- Bis(tri-n-butyltin)oxide (TBTO) poses a significant risk to maternal health and embryonic development.
- Organ weight changes in spleen and placenta indicate potential toxicological mechanisms of TBTO.
- Further research is warranted to elucidate the specific pathways of TBTO-induced developmental toxicity.