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Dexamethasone inhibits U937 cell adhesion via the down-regulation of ROCK1 activity
Dong Liu1, Xing-Yun Chen, Ren-Ping Xiong
1Molecular Biology Center, State Key Laboratory of Trauma, Burn, and Combined Injury, Research Institute of Surgery and Daping Hospital, Third Military Medical University, Chongqing 400042, China.
Objective:
To explore the effect of dexamethasone (DEX) on monocyte adhesion function and its underlying mechanism.
Methods:
The effects of DEX and fasudil on adhesion of cultured U937 monocytes to human umbilical vein endothelial cells (HUVEC) following stimulation with phorbol myristate acetate (PMA) were studied; Changes in the Rho-associated coiled-coil protein kinase 1 (ROCK1) protein content and activity were evaluated.
Results:
DEX and fasudil significantly inhibited U937 cell adhesion rates under PMA stimulation and inhibited ROCK1 activity. Mifepristone (RU-486) and cycloheximide (CHX) did not alter these effects of DEX.
Conclusions:
DEX interferes with the adhesion function of U937 cells through the inhibition of ROCK1 activity.
