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Updated: May 17, 2026

Ratiometric Imaging of Extracellular pH in Dental Biofilms
Published on: March 9, 2016
The acidogenic potential of different milk formulas on dental plaque pH
Ananda S Raju1, Mythri Hirehal, Prashant G Manjunath
1Department of Community Dentistry, Sri Hasanamba Dentla College, Hassan, Karnataka 573201, India. dr.anandsr@yahoo.co.in
Insights
Infant milk formulas significantly reduce dental plaque pH, increasing the risk of early childhood caries (ECC). Parents and clinicians should be aware of these effects when choosing formulas.
Area of Science:
- Pediatric Dentistry
- Dental Public Health
- Nutritional Science
Background:
- Dental plaque pH is a critical factor in the demineralization-remineralization balance of tooth enamel.
- Early Childhood Caries (ECC) is a prevalent oral health issue in young children, influenced by diet.
- Infant formulas are a primary dietary source for many young children.
Purpose of the Study:
- To evaluate the impact of different commercial infant milk formulas on dental plaque pH.
- To compare the buffering capacity of various infant formulas.
- To assess the cariogenic potential of infant formulas based on their effect on plaque pH.
Main Methods:
- Six commercial infant milk formulas were tested on ten children aged 7-10 years.
- Plaque pH was measured using a combination electrode after rinsing with formulas, sucrose solution (positive control), and deionized water (negative control).
- Statistical analysis included Tukey's multiple comparison test to determine significant differences (P < 0.05).
Main Results:
- All tested infant formulas significantly reduced plaque pH below pre-rinse levels and water control.
- Each formula exhibited a variable buffering effect, with Lactodex showing the highest (20.13%) and Nestogen the lowest (9.21%).
- The observed pH drop ranged from 0.72 (Farex) to 0.98 (Nan).
Conclusions:
- Infant formulas can significantly lower dental plaque pH, contributing to the etiology of ECC.
- Awareness of formula-specific effects on pH is crucial for parents and clinicians.
- Findings may inform the development of more tooth-friendly infant food options.
Purpose:
To investigate the effect of different milk formulas on dental plaque pH.
Materials And Methods:
Six commercial infant milk formulas were selected, with 10% sucrose solution as the positive and deionised water as the negative control, and tested among ten children aged 7-10 years. The plaque pH changes were recorded by using a combination electrode.
Results:
Mouth rinsing with all infant formulas significantly reduced the plaque pH (Tukey's multiple comparison test, P < 0.05) to levels below the pre-rinse pH value for sucrose solution and also below the pH obtained after rinsing with water. Each milk formula had a significant but variable buffering effect or tendency to decrease the pH. The formula with the least buffering effect was Nestogen (9.21%) followed by Nan (11.92%), and the highest buffering effect was shown by Lactodex (20.13%). The pH drop ranged from 0.72 for Farex to 0.98 for Nan.
Conclusion:
The results of this study underscore the need for parents and clinicians to be fully aware of the potential harm of various infant formulas and their major role in the etiology of ECC. These findings may help in creating a new generation of foods which, if not 'friendly to teeth', may be thought of as 'friendlier' than their predecessors.
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