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Related Experiment Video

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Procedure to Evaluate the Efficiency of Flocculants for the Removal of Dispersed Particles from Plant Extracts

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Nanofiber adsorbents for high productivity downstream processing.

Oliver Hardick¹, Stewart Dods, Bob Stevens

¹Department of Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, United Kingdom.

Biotechnology and Bioengineering

|October 26, 2012

Summary

This summary is machine-generated.

Diethylaminoethyl (DEAE) derivatized cellulose nanofibers offer higher flow and mass transfer for bioseparations. These novel adsorbents show potential for a 10-fold productivity increase in chromatography despite lower binding capacity.

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Area of Science:

Biotechnology
Materials Science
Chemical Engineering

Background:

Electrospun polymeric nanofibers provide high surface area for ligand support in bioseparations.
Conventional chromatography methods face limitations in purification productivity.

Purpose of the Study:

To investigate the performance of diethylaminoethyl (DEAE) derivatized regenerated cellulose nanofiber adsorbents for bioseparation.
To evaluate mass transfer, flow properties, binding capacity, and fouling effects.

Main Methods:

Fabrication of DEAE-derivatized cellulose nanofibers into a 0.15 mL cartridge.
Assessment of flow properties, binding capacity using Bovine Serum Albumin (BSA), and fouling resistance with yeast homogenate.
Evaluation of performance under harsh cleaning-in-place conditions over multiple cycles.

Main Results:

Nanofiber adsorbents exhibited significantly higher flow and mass transfer properties compared to packed beds.
DEAE-nanofiber adsorbents showed 10% of packed bed binding capacity for BSA but operated at 100x higher flow rates, suggesting a 10-fold productivity increase.
The novel adsorbent material demonstrated reproducible performance with complex feed material and harsh cleaning cycles, showing superior permeability and fouling resistance.

Conclusions:

DEAE-derivatized cellulose nanofibers represent a promising alternative adsorbent material for bioseparation applications.
Their enhanced flow and mass transfer properties, coupled with good fouling resistance, indicate potential for improved purification productivity in chromatography.