Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Clinical Applications of Epidermal Stem Cells

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own EpiSCs...

Cells of the Epidermis

Cells of the Epidermis

The epidermis is made of four or five layers of epithelial cells, depending on its location in the body. From deep to superficial, these layers are the stratum basale, stratum spinosum, stratum granulosum, stratum lucidum, and stratum corneum.
The cells in all these layers except the stratum basale are called keratinocytes, a type of cell that manufactures and stores the protein keratin. The keratinocytes in the stratum corneum are dead and regularly slough away, being replaced by cells from...

Renewal of Skin Epidermal Stem Cells

Renewal of Skin Epidermal Stem Cells

The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular cells,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The viable bioengineered allogeneic cellularized construct StrataGraft® synthesizes, deposits, and organizes human extracellular matrix proteins into tissue type-specific structures and secretes soluble factors associated with wound healing.

Burns : journal of the International Society for Burn Injuries·2023

Same author

Assessment of Low Vision Referrals before and after Establishment of a Low Vision Program at an Academic Medical Center.

Optometry and vision science : official publication of the American Academy of Optometry·2023

Same author

A phase 3, open-label, controlled, randomized, multicenter trial evaluating the efficacy and safety of StrataGraft® construct in patients with deep partial-thickness thermal burns.

Burns : journal of the International Society for Burn Injuries·2021

Same author

Control of Pain in Intravitreal Injections: Evaluation of Combination Topical Anesthetics and Non-steroidal Anti-inflammatory Drops: A Randomized, Placebo-Controlled Clinical Trial.

Ophthalmology. Retina·2020

Same author

An open-label, prospective, randomized, controlled, multicenter, phase 1b study of StrataGraft skin tissue versus autografting in patients with deep partial-thickness thermal burns.

Burns : journal of the International Society for Burn Injuries·2019

Same author

Chimeric autologous/allogeneic constructs for skin regeneration.

Military medicine·2014

Search research articles

Related Experiment Video

Updated: May 17, 2026

Generation and Culturing of Primary Human Keratinocytes from Adult Skin

Generation and Culturing of Primary Human Keratinocytes from Adult Skin

Published on: December 22, 2017

Classical human epidermal keratinocyte cell culture.

Cathy Rasmussen¹, Christina Thomas-Virnig, B Lynn Allen-Hoffmann

¹Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA.

Methods in Molecular Biology (Clifton, N.J.)

|October 26, 2012

Summary

This study details methods for culturing human keratinocytes and dermal fibroblasts. These protocols enable the generation of stratified skin tissue for research and therapeutic applications.

More Related Videos

Isolation and Culture of Adult Epithelial Stem Cells from Human Skin

Isolation and Culture of Adult Epithelial Stem Cells from Human Skin

Published on: March 31, 2011

Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity

Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity

Published on: January 30, 2021

Related Experiment Videos

Last Updated: May 17, 2026

Generation and Culturing of Primary Human Keratinocytes from Adult Skin

Generation and Culturing of Primary Human Keratinocytes from Adult Skin

Published on: December 22, 2017

Isolation and Culture of Adult Epithelial Stem Cells from Human Skin

Isolation and Culture of Adult Epithelial Stem Cells from Human Skin

Published on: March 31, 2011

Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity

Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity

Published on: January 30, 2021

Area of Science:

Dermatology
Cell Biology
Tissue Engineering

Background:

Serial cultivation of human keratinocytes was established over 30 years ago.
Early methods relied on murine fibroblast feeder layers for keratinocyte propagation.
Advances have expanded techniques for both 2D monolayer and 3D organotypic cultures.

Purpose of the Study:

To provide protocols for the isolation and serial cultivation of primary human keratinocytes and dermal fibroblasts.
To guide the generation of stratified skin tissue using these cell types.
To document traditional culturing methods for keratinocytes.

Main Methods:

Isolation of primary keratinocytes from human skin tissue.
Serial cultivation using traditional monolayer and organotypic culture techniques.
Co-cultivation of keratinocytes and dermal fibroblasts for stratified tissue generation.

Main Results:

Established protocols for keratinocyte and fibroblast isolation and expansion.
Demonstrated the utility of monolayer culture for keratinocyte proliferation.
Showcased organotypic culture for promoting keratinocyte differentiation and stratification.

Conclusions:

Traditional methods for human keratinocyte and fibroblast culture remain valuable.
These protocols facilitate the creation of functional, stratified skin tissue.
The described techniques support advancements in skin research and regenerative medicine.