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Related Experiment Video

Updated: May 17, 2026

Mixed Primary Cultures of Murine Small Intestine Intended for the Study of Gut Hormone Secretion and Live Cell Imaging of Enteroendocrine Cells

Mixed Primary Cultures of Murine Small Intestine Intended for the Study of Gut Hormone Secretion and Live Cell Imaging of Enteroendocrine Cells

Published on: April 20, 2017

Primary mouse small intestinal epithelial cell cultures.

Toshiro Sato¹, Hans Clevers

¹Department of Gastroenterology, School of Medicine, Keio University, Tokyo, Japan. T.sato@a7.keio.jp

Methods in Molecular Biology (Clifton, N.J.)

|October 26, 2012

Summary

This summary is machine-generated.

Researchers developed a robust method to culture primary intestinal stem cells. These cells form organoids, mimicking the in vivo intestinal epithelium, aiding stem cell research.

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Last Updated: May 17, 2026

Mixed Primary Cultures of Murine Small Intestine Intended for the Study of Gut Hormone Secretion and Live Cell Imaging of Enteroendocrine Cells

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Published on: April 20, 2017

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Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells

Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells

Published on: March 23, 2022

Area of Science:

Gastroenterology
Stem Cell Biology
Epithelial Biology

Background:

The intestinal epithelium is a highly regenerative tissue.
Identifying and culturing intestinal stem cells has been challenging.
Previous culture systems lacked reproducibility and robustness.

Purpose of the Study:

To establish a defined, reproducible, and robust culture system for primary small intestinal epithelial stem cells.
To screen for optimal growth factor combinations regulating intestinal stem cell self-renewal and differentiation.
To utilize genetic evidence for optimizing culture conditions.

Main Methods:

Screening of optimal growth factor combinations.
Isolation of primary small intestinal epithelial stem cells.
Culture of isolated crypts and single Lgr5+ stem cells.

Main Results:

Established a culture system where isolated crypts form 'organoid structures'.
Organoid structures recapitulate the histological hierarchy of the in vivo intestinal epithelium.
Single Lgr5+ intestinal stem cells form organoids, maintaining self-renewal and differentiating into all epithelial lineages.

Conclusions:

Developed a robust method for culturing primary intestinal stem cells.
The established culture system allows for the study of intestinal stem cell self-renewal and differentiation.
This system provides a valuable tool for investigating intestinal epithelial biology.